Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

pT7
BBa_K525998 Version 1 (Component)
Promoter T7 and RBS

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_K1991009
BBa_K1991009 Version 1 (Component)
Pcons-RBS-LO-AOX2-His

T7 RBS
BBa_K1362090 Version 1 (Component)
strong T7 RBS

BBa_K1991006
BBa_K1991006 Version 1 (Component)
LO-AOX2-His

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

BBa_K1991008
BBa_K1991008 Version 1 (Component)
Pcons-RBS-LO-AOX1-His

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

BBa_C0261
BBa_C0261 Version 1 (Component)
AHL-making Enzyme, luxI (+RBS)

RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop

RBS-spoVG
BBa_K143021 Version 1 (Component)
SpoVG ribosome binding site (RBS) for B. subtilis

BBa_K1991010
BBa_K1991010 Version 1 (Component)
Pcons-RBS-LO-GFP-His

BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS_rev) -- corresponds to BBa_B0030

BBa_K1991005
BBa_K1991005 Version 1 (Component)
LO-AOX1-His (Lpp-OmpA-Alcohol Oxidase)

BBa_E0430
BBa_E0430 Version 1 (Component)
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)

BBa_K1991003
BBa_K1991003 Version 1 (Component)
Pcons-RBS-AOX2

BBa_K1991007
BBa_K1991007 Version 1 (Component)
Pcons-RBS-LO-BamHI

RBS_cassette
RBS_cassette Version 1 (Component)
AvrII restriction site (cctagg), overlapped with RBS (aggagga)

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

eyfp
BBa_E0031 Version 1 (Component)
EYFP (RBS+ LVA-)

BBa_K165002
BBa_K165002 Version 1 (Component)
Kozak sequence (yeast RBS)

Hin
BBa_J31000 Version 1 (Component)
DNA-invertase Hin from Salmonella typhimurium

His
BBa_K157011 Version 1 (Component)
His affinity tag; Freiburg standard

pCon 0.36
BBa_K176009 Version 1 (Component)
constitutive promoter family member J23107 actual sequence (pCon 0.36)

RE
BBa_J3101 Version 1 (Component)
Recombinational Enhancer (RE) for Hin/Hix inverting

pCon 0.15
BBa_K176008 Version 1 (Component)
constitutive promoter family member J23115 actual sequence (pCon 0.15)

BBa_K1491009
BBa_K1491009 Version 1 (Component)
RBS-mRFP-His

RBS-His-Sc
BBa_K1602031 Version 1 (Component)
HisTag-Scaffold_Si4-Tag fusion protein with RBS BBa_B0034

BBa_B0053
BBa_B0053 Version 1 (Component)
Terminator (His)

ecfp
BBa_E0021 Version 1 (Component)
ECFP (RBS+ LVA-)

BBa_K1491005
BBa_K1491005 Version 1 (Component)
RBS-SuperYFP2-His

BBa_K1991002
BBa_K1991002 Version 1 (Component)
Pcons-RBS-AOX1 (Alcohol Oxidase)