Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

pSB1C3
pSB1C3 Version 1 (Component)
High copy BioBrick assembly plasmid

BBa_J04450
BBa_J04450 Version 1 (Component)
RFP Coding Device

Scar 25
BBa_B0105 Version 1 (Component)
RFC 25 Scar Sequence

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

GFP report
BBa_E0240 Version 1 (Component)
GFP generator

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

SEGA021
SEGA021 Version 1 (Component)
natural linear DNA.

1GateNpuE
BBa_K1362101 Version 1 (Component)
RBS+NpuDnaE C-intein RFC[105] assembly construct (with His6)

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

SEGA022
SEGA022 Version 1 (Component)
natural linear DNA.

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

1Gate-NpuD
BBa_K1362100 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] assembly construct (with His6)

RFC 37
BBa_K245009 Version 1 (Component)
RFC 37 Scar Sequence

1NpuE(C*)
BBa_K1362103 Version 1 (Component)
RBS + NpuDnaE C-intein RFC[105] non-slicing control construct (with His6)

tetA(C)f
BBa_J31007 Version 1 (Component)
tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]

RFC105 Z
BBa_K1362421 Version 1 (Component)
C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z

NpuDnaE(C)
BBa_K1362401 Version 1 (Component)
NpuDnaE C-Intein cloning piece

TerDnaE3(C
BBa_K1362131 Version 1 (Component)
RBS + TerDnaE3 C-intein RFC[105] assembly construct (with His6)

SspDnaB(C)
BBa_K1362141 Version 1 (Component)
RBS + SspDnaB C-intein RFC[105] assembly construct (with His6)

SspGyrB(C)
BBa_K1362121 Version 1 (Component)
RBS + SspGyrB C-intein RFC[105] assembly construct (with His6)

BBa_K1362111
BBa_K1362111 Version 1 (Component)
RBS + SspDnaX C-intein RFC[105] assembly construct (with His6)

mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

CircNpu
BBa_K1362000 Version 1 (Component)
NpuDnaE intein RFC[105] circularization construct

GFP genera
BBa_E0840 Version 1 (Component)
GFP generator

1NpuE(N*)
BBa_K1362102 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] non-splicing control construct (with His6)