SynBioHub

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SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

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BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

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SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

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BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

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BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

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BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

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BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

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BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

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BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

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pT7
BBa_K525998 Version 1 (Component)
Promoter T7 and RBS

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T7 RBS
BBa_K1362090 Version 1 (Component)
strong T7 RBS

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RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

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BBa_C0261
BBa_C0261 Version 1 (Component)
AHL-making Enzyme, luxI (+RBS)

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//rbs
rbs Version 1 (Collection)

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RBS-spoVG
BBa_K143021 Version 1 (Component)
SpoVG ribosome binding site (RBS) for B. subtilis

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RBS_SpaR
RBS_SpaR Version (Component)

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RBS_SpaK
RBS_SpaK Version (Component)

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BBa_J52008
BBa_J52008 Version 1 (Component)
luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)

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BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS<sub>rev</sub>) -- corresponds to BBa_B0030

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RBS_SpaR_seq
RBS_SpaR_seq Version (Sequence)

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RBS_SpaK_seq
RBS_SpaK_seq Version (Sequence)

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BBa_E0430
BBa_E0430 Version 1 (Component)
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)

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RBS_cassette
RBS_cassette Version 1 (Component)
AvrII restriction site (cctagg), overlapped with RBS (aggagga)

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BBa_K1114107
BBa_K1114107 Version 1 (Component)
Bicistronic Design RBS 2, MoClo Format with BC fusion sites

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eyfp
BBa_E0031 Version 1 (Component)
EYFP (RBS+ LVA-)

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BBa_K165002
BBa_K165002 Version 1 (Component)
Kozak sequence (yeast RBS)

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PBS_F24
PBS_F24 Version 1 (Component)

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ecfp
BBa_E0021 Version 1 (Component)
ECFP (RBS+ LVA-)

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RBS-GFP-Te
BBa_I763020 Version 1 (Component)
RBS-GFP (+LVA Tag)-Terminators

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rbs+crtZ
BBa_I742158 Version 1 (Component)
crtZ (beta-carotene hydroxylase) with native rbs

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BBa_K608002
BBa_K608002 Version 1 (Component)
strong Promoter and strong RBS

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BBa_E0422
BBa_E0422 Version 1 (Component)
ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)

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RBS_SpaS
RBS_SpaS Version (Component)

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BBa_E0420
BBa_E0420 Version 1 (Component)
ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)

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BBa_K199118
BBa_K199118 Version 1 (Component)
T7 promoter + RBS + RFP

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scar
BBa_G0000 Version 1 (Component)
SpeI/XbaI scar for RBS-CDS junctions

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BBa_B0035
BBa_B0035 Version 1 (Component)
RBS (B0030 derivative)

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SYFP2
BBa_K864100 Version 1 (Component)
Super Yellow Fluorescent Protein 2 (SYFP2)

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BBa_K880005
BBa_K880005 Version 1 (Component)
Strong promoter, strong RBS combination for high expression levels of proteins

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mCherry
BBa_J06702 Version 1 (Component)
mCherry, bacterial with RBS and forward terminator

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BBa_K081005
BBa_K081005 Version 1 (Component)
constitutive promoter family member and RBS

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RBS-GsiB
BBa_K143020 Version 1 (Component)
GsiB ribosome binding site (RBS) for B. subtilis

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BBa_K1351029
BBa_K1351029 Version 1 (Component)
LMU Bacillus RBS collection 2

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BBa_B0073
BBa_B0073 Version 1 (Component)
Specialized RBS

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RBS_SpaS_seq
RBS_SpaS_seq Version (Sequence)

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BBa_K117002
BBa_K117002 Version 1 (Component)
LsrA promoter (indirectly activated by AI-2)

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BBa_M300056
BBa_M300056 Version 1 (Component)
gene 2 rbs

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rbs+LIMS1
BBa_I742111 Version 1 (Component)
Limonene synthase gene with ribosome binding site

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BBa_K1799028
BBa_K1799028 Version 1 (Component)
RBS.2

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BBa_K1763442
BBa_K1763442 Version 1 (Component)
MaSp1/2[1:2]-12(1C3)

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