Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
pSB1C3pSB1C3 Version 1 (Component)High copy BioBrick assembly plasmid
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
BBa_J64101BBa_J64101 Version 1 (Component)ColE1 biobrick plasmid with backward terminator
EcoRIEcoRI Version 1 (Component) NheINheI Version 1 (Component) PacIPacI Version 1 (Component) SpeISpeI Version 1 (Component) BBa_I13450BBa_I13450 Version 1 (Component)Removes BioBrick End
pT7BBa_K525998 Version 1 (Component)Promoter T7 and RBS
cI+luxRBBa_R0065 Version 1 (Component)Promoter (lambda cI and luxR regulated -- hybrid)
pSB1AT3pSB1AT3 Version 1 (Component)High copy BioBrick assembly plasmid
BBa_I13452BBa_I13452 Version 1 (Component)Adds another BioBrick site
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
BBa_I13451BBa_I13451 Version 1 (Component)Removes left Biobrick end
PrefixBBa_G00000 Version 1 (Component)BioBrick cloning site prefix
SuffixBBa_G00001 Version 1 (Component)BioBrick cloning site suffix
BBa_K1321300BBa_K1321300 Version 1 (Component)pSEVA331-Bb - pSEVA331 with biobrick prefix and suffix
BBa_K299107BBa_K299107 Version 1 (Component)T.xbaI.G (T followed by xbaI site and additional G to behave like biobrick prefix after xbaI digest)
F plasmidBBa_I50000 Version 1 (Component)F plasmid backbone with BioBrick sites removed.
BBa_I50001BBa_I50001 Version 1 (Component)F plasmid backbone with BioBrick sites removed, reverse
BBa_J33207BBa_J33207 Version 1 (Component)lac promoter and lacZ
BBa_J64100BBa_J64100 Version 1 (Component)Medium copy ColE1 BioBrick vector
BBa_K189000BBa_K189000 Version 1 (Component)Targeted BioBrick
VF2BBa_G00100 Version 1 (Component)Forward primer for sequencing/amplifying BioBrick parts (VF2)
VRBBa_G00102 Version 1 (Component)Reverse BioBrick primer annealing site (VR binding site)
BBa_K1559011BBa_K1559011 Version 1 (Component)Low-copy plasmid pTRC99A (trc promoter and lacI removed)
pSB1AK3pSB1AK3 Version 1 (Component)High copy BioBrick assembly plasmid
rhIIBBa_C0070 Version 1 (Component)autoinducer synthetase for N-butyryl-HSL (BHL) and HHL
BBa_K608002BBa_K608002 Version 1 (Component)strong Promoter and strong RBS
BBa_I11031BBa_I11031 Version 1 (Component)excisionase from E. coli phage P22 (removes prophage from host genome)
BBa_I11021BBa_I11021 Version 1 (Component)excisionase from E. coli phage lambda (removes prophage from host genome)
BBa_J61016BBa_J61016 Version 1 (Component)Biobrick converter for BamHI-part-EcoRI
BBa_K1162011BBa_K1162011 Version 1 (Component)GFP with stop codon removed
mCherryBBa_J06702 Version 1 (Component)mCherry, bacterial with RBS and forward terminator
BBa_K228009BBa_K228009 Version 1 (Component)AraC protein(reversed sequence) and Pbad promoter
BBa_J70012BBa_J70012 Version 1 (Component)A BioScaffold Part (Uses PsrI), Protein Head Remover see Part Design page
BBa_K299802BBa_K299802 Version 1 (Component)Aactag - like a biobrick scar but first T changed to A
BBa_K081005BBa_K081005 Version 1 (Component)constitutive promoter family member and RBS
cinRBBa_R0078 Version 1 (Component)Promoter (cinR and HSL regulated)
BioBrick-rBBa_G1005 Version 1 (Component)Reverse BioBrick suffix primer for amplifying BioBrick parts (BioBrick-r)
BioBrick-fBBa_G1004 Version 1 (Component)Forward BioBrick prefix primer for amplifying BioBrick parts (BioBrick-f)
GC Optimised Sequence PullGC_Optimised Version 1 (Activity)Used to indicate that a sequence was GC optimised after pulling from the source and before submission