BBF10K_003498_backbone
BBF10K_003498_backbone Version 1 (Unknown)
synthetic DNA

iGEM Feature Libraries
igem_feature_libraries_collection Version 1 (Unknown)
A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Free Genes Feature Libraries
free_genes_feature_libraries_collection Version 1 (Unknown)
A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

pSB1C3
pSB1C3 Version 1 (Component)
High copy BioBrick assembly plasmid

lux pR
BBa_R0062 Version 1 (Component)
Promoter (luxR & HSL regulated -- lux pR)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

luxI
BBa_C0061 Version 1 (Component)
autoinducer synthetase for AHL

BBa_J64101
BBa_J64101 Version 1 (Component)
ColE1 biobrick plasmid with backward terminator

Prefix-R
BBa_G1001 Version 1 (Component)
Reverse primer for amplifying BioBrick plasmid backbones by PCR (Prefix-r)

Prefix-R
BBa_G1003 Version 1 (Component)
Reverse primer for amplifying BioBrick plasmid backbones by PCR (Prefix-r)

BBa_B0025
BBa_B0025 Version 1 (Component)
double terminator (B0015), reversed

pSC101
BBa_I50041 Version 1 (Component)
pSC101 origin of replication, reverse

VF2
BBa_G00100 Version 1 (Component)
Forward primer for sequencing/amplifying BioBrick parts (VF2)

OriTR
BBa_J01003 Version 1 (Component)
OriT-R (Origin of transfer for the R-plasmid nic region)

mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

Suffix-F
BBa_G1000 Version 1 (Component)
Forward primer for amplifying BioBrick plasmid backbones by PCR (Suffix-f)

Suffix-F
BBa_G1002 Version 1 (Component)
Forward primer for amplifying BioBrick plasmid backbones by PCR (Suffix-f)

Prefix
BBa_G00000 Version 1 (Component)
BioBrick cloning site prefix

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

BioBrick-r
BBa_G1005 Version 1 (Component)
Reverse BioBrick suffix primer for amplifying BioBrick parts (BioBrick-r)

VR
BBa_G00102 Version 1 (Component)
Reverse BioBrick primer annealing site (VR binding site)

BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS_rev) -- corresponds to BBa_B0030

pSB1AT3
pSB1AT3 Version 1 (Component)
High copy BioBrick assembly plasmid

BBa_K299107
BBa_K299107 Version 1 (Component)
T.xbaI.G (T followed by xbaI site and additional G to behave like biobrick prefix after xbaI digest)

mRFPr
BBa_J31008 Version 1 (Component)
RFP reverse

BioBrick-f
BBa_G1004 Version 1 (Component)
Forward BioBrick prefix primer for amplifying BioBrick parts (BioBrick-f)

VR
BBa_G00101 Version 1 (Component)
Reverse primer for sequencing/amplifying BioBrick parts (VR)

BBa_I13507
BBa_I13507 Version 1 (Component)
Screening plasmid intermediate

BBa_K1321300
BBa_K1321300 Version 1 (Component)
pSEVA331-Bb - pSEVA331 with biobrick prefix and suffix

BBa_I13452
BBa_I13452 Version 1 (Component)
Adds another BioBrick site

BBa_B0024
BBa_B0024 Version 1 (Component)
double terminator (B0012-B0011), reversed

luxI
BBa_C0161 Version 1 (Component)
autoinducer synthetase for AHL (no LVA)

BBa_K648013
BBa_K648013 Version 1 (Component)
GFP with Standard 25 Prefix/Suffix

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

BBa_K371049
BBa_K371049 Version 1 (Component)
MPF(meta-prefix)

BBa_J44002
BBa_J44002 Version 1 (Component)
pBAD reverse

BBa_I13504
BBa_I13504 Version 1 (Component)
Screening plasmid intermediate