CnU6
CnU6_terminator Version 1 (Unknown)
Organism: Cryptococcus neoformans var. neoformans JEC21

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_B0015
BBa_B0015 Version 1 (Component)
double terminator (B0010-B0012)

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

BBa_B0011
BBa_B0011 Version 1 (Component)
LuxICDABEG (+/-)

BBa_B1001
BBa_B1001 Version 1 (Component)
Terminator (artifical, small, %T~=90)

Composite Sequence Pull
Composite Version 1 (Activity)
When multiple sources where combined to make a new part.

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

NLS+Term
BBa_K165018 Version 1 (Component)
SV40 nuclear localization sequence + ADH1 transcription terminator

BBa_M36010
BBa_M36010 Version 1 (Component)
Transcription Terminator (Strong)

Scar 25
BBa_B0105 Version 1 (Component)
RFC 25 Scar Sequence

BBa_B0014
BBa_B0014 Version 1 (Component)
double terminator (B0012-B0011)

LacI
BBa_R0010 Version 1 (Component)
promoter (lacI regulated)

BBa_B0025
BBa_B0025 Version 1 (Component)
double terminator (B0015), reversed

BBa_K165010
BBa_K165010 Version 1 (Component)
ADH1 yeast transcription terminator

BBa_J56012
BBa_J56012 Version 1 (Component)
Invertible sequence of dna includes Ptrc promoter

BBa_K801012
BBa_K801012 Version 1 (Component)
ADH1 yeast terminator

BBa_B1006
BBa_B1006 Version 1 (Component)
Terminator (artificial, large, %T~>90)

BBa_B0016
BBa_B0016 Version 1 (Component)
Terminator (T7 RNAP specific, T_Phi)

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

SV40 NLS
BBa_J63008 Version 1 (Component)
SV40 nuclear localization sequence from SV40; yeast codon optimized

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

scar
BBa_K112999 Version 1 (Component)
BBb1 scar sequence

BBa_K1051006
BBa_K1051006 Version 1 (Component)
Saccharomycescerevisiae TBY-1 terminator with stopcodon

BBa_I712028
BBa_I712028 Version 1 (Component)
CherryNLS - synthetic construct monomeric red fluorescent protein with nuclear localization sequence

BBa_K392003
BBa_K392003 Version 1 (Component)
yeast ADH1 terminator

BBa_B0024
BBa_B0024 Version 1 (Component)
double terminator (B0012-B0011), reversed

RFC 37
BBa_K245009 Version 1 (Component)
RFC 37 Scar Sequence

BBa_K1150012
BBa_K1150012 Version 1 (Component)
BGH terminator