iGEM Feature Libraries
igem_feature_libraries_collection Version 1 (Unknown)
A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Free Genes Feature Libraries
free_genes_feature_libraries_collection Version 1 (Unknown)
A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_K1983008
BBa_K1983008 Version 1 (Component)
Streptococcus thermofilus Csm4 phenylalanine mutant M27 with C-terminal 6XHis-Tag

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

BBa_K1983007
BBa_K1983007 Version 1 (Component)
Streptococcus thermofilus Csm4 phenylalanine mutant M11 with C-terminal 6XHis-Tag

BBa_K1983009
BBa_K1983009 Version 1 (Component)
Streptococcus thermofilus Csm4 phenylalanine mutant M42 with C-terminal 6XHis-Tag

BBa_K249006
BBa_K249006 Version 1 (Component)
Fusion EYFP with C-terminal Arginine Tag

tetA(C)f
BBa_J31007 Version 1 (Component)
tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]

BBa_K1983005
BBa_K1983005 Version 1 (Component)
gp45 phenylalanine mutant M25 with C-terminal 6XHis-Tag

BBa_K1983006
BBa_K1983006 Version 1 (Component)
gp45 phenylalanine mutant M37 with C-terminal 6XHis-Tag

BBa_K249008
BBa_K249008 Version 1 (Component)
YFP with N-terminal Arganine Tag

BBa_K1321340
BBa_K1321340 Version 1 (Component)
Double CBD (dCBD) with N-terminal linker

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

BBa_K331002
BBa_K331002 Version 1 (Component)
ECFP with C-terminal Arginine Tag

BBa_K1983003
BBa_K1983003 Version 1 (Component)
gp45 phenylalanine mutant M11 with C-terminal 6XHis-Tag

BBa_K1983004
BBa_K1983004 Version 1 (Component)
gp45 phenylalanine mutant M15 with C-terminal 6XHis-Tag

BBa_K1983002
BBa_K1983002 Version 1 (Component)
gp45 phenylalanine mutant M6 with C-terminal 6XHis-Tag

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

BBa_K1333005
BBa_K1333005 Version 1 (Component)
M13 bacteriophage g8p with a FLAG tag on C-terminal

BBa_J04031
BBa_J04031 Version 1 (Component)
GFP w/LVA tag

RFC105 Z
BBa_K1362421 Version 1 (Component)
C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z

tetA(C)b
BBa_J31006 Version 1 (Component)
tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]

BBa_K1100006
BBa_K1100006 Version 1 (Component)
optimized sfGFP with Csy4 insulator loci

BBa_I13458
BBa_I13458 Version 1 (Component)
promoter C and arabinose C operon

HA
BBa_I712008 Version 1 (Component)
HA affinity tag

BBa_K1333002
BBa_K1333002 Version 1 (Component)
M13 bacteriophage g3p with start codon of "ATG" and a FLAG tag on C-terminal

NLS
BBa_J176008 Version 1 (Component)
NLS

BBa_K105012
BBa_K105012 Version 1 (Component)
10 aa flexible protein domain linker

PEST
BBa_J176018 Version 1 (Component)
PEST

BBa_K331001
BBa_K331001 Version 1 (Component)
ECFP with N-terminal Arginine Tag

BBa_J64101
BBa_J64101 Version 1 (Component)
ColE1 biobrick plasmid with backward terminator

BBa_K1983000
BBa_K1983000 Version 1 (Component)
Codon optimized AvPAL with C-terminal 6XHis-Tag

CBDcipA
BBa_K1615111 Version 1 (Component)
CBDcipA with N and C-terminal linker in RFC25

linker
BBa_K133132 Version 1 (Component)
8 aa protein domain linker

RFC105 A
BBa_K1362414 Version 1 (Component)
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A

BBa_K1321014
BBa_K1321014 Version 1 (Component)
CBDCipA with N and C-terminal linker

G-His6(N)
BBa_K1362430 Version 1 (Component)
C-terminal His6 (N-Intein)

BBa_J04051
BBa_J04051 Version 1 (Component)
RFP with tag

lacZ a
BBa_E0033 Version 1 (Component)
LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)

NLSmycSTOP
BBa_J176031 Version 1 (Component)
NLS myc stop

RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop