Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
iGEM Feature Librariesigem_feature_libraries_collection Version 1 (Unknown)A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Free Genes Feature Librariesfree_genes_feature_libraries_collection Version 1 (Unknown)A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
BBa_B0010BBa_B0010 Version 1 (Component)T1 from E. coli rrnB
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
BBa_B0012BBa_B0012 Version 1 (Component)TE from coliphageT7
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
lacIBBa_C0012 Version 1 (Component)lacI repressor from E. coli (+LVA)
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
cI lamBBa_C0051 Version 1 (Component)cI repressor from E. coli phage lambda (+LVA)
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
ATGATG Version 1 (Component) BBa_J15001BBa_J15001 Version 1 (Component)strong synthetic E. coli ribosome binding site with SacI site.
BBa_K844000BBa_K844000 Version 1 (Component)10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
BBa_K1051208BBa_K1051208 Version 1 (Component)The degradation tag in E. coil ,M0052 with TAATAA.
BBa_J04500BBa_J04500 Version 1 (Component)IPTG inducible promoter with RBS
BBa_K2066527BBa_K2066527 Version 1 (Component)B0034 with scar TACTAG
Scar25 ATGBBa_K1319106 Version 1 (Component)RFC 25 Translation Start Scar Sequence
BBa_K1051206BBa_K1051206 Version 1 (Component)The degradation tag in E. coil ,M0050 with TAATAA.
BBa_K1051207BBa_K1051207 Version 1 (Component)The degradation tag in E. coil ,M0051 with TAATAA.
araCBBa_C0080 Version 1 (Component)araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
HisTagBBa_J18909 Version 1 (Component)His tag (6xHis; E. coli - optimized)
cI HK022BBa_C0050 Version 1 (Component)cI repressor from phage HK022 (+LVA?)
His-StopBBa_K133133 Version 1 (Component)His affinity tag-stop
cI HK022BBa_R0050 Version 1 (Component)Promoter (HK022 cI regulated)
LAABBa_M0050 Version 1 (Component)AANDENYALAA. (Very fast) SsrA degradation tag.
BBa_J04031BBa_J04031 Version 1 (Component)GFP w/LVA tag
BBa_M0051BBa_M0051 Version 1 (Component)AANDENYNYADAS. (Fast) SsrA degradation tag.
BBa_J33201BBa_J33201 Version 1 (Component)E. coli chromosomal ars promoter with arsR repressor gene
BBa_K1399005BBa_K1399005 Version 1 (Component)GFP (mut3b) with AAV-ssrA degradation tag
BBa_K1399004BBa_K1399004 Version 1 (Component)GFP (mut3b) with LVA-ssrA degradation tag
BBa_J70029BBa_J70029 Version 1 (Component)RBS + mCherry gene, optimized for expression in Me. florum and E. coli
HABBa_I712008 Version 1 (Component)HA affinity tag
NLSBBa_J176008 Version 1 (Component)NLS
BBa_J70594BBa_J70594 Version 1 (Component)RFC12 TAATAA Tail Domain
BBa_K1399007BBa_K1399007 Version 1 (Component)GFP (mut3b) with SsrA-DAS+2 degradation tag
BBa_K105012BBa_K105012 Version 1 (Component)10 aa flexible protein domain linker
PESTBBa_J176018 Version 1 (Component)PEST
PelBBBa_J32015 Version 1 (Component)PelB leader sequence; directs protein to E. coli periplasmic membrane
BBa_K654058BBa_K654058 Version 1 (Component)Thioesterase (TesA from E. coli) with 8-His Tag