Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

mCh
BBa_J176005 Version 1 (Component)
mCh

fshPCD
BBa_J176002 Version 1 (Component)
fshPCD

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

VP64
BBa_J176013 Version 1 (Component)
VP64

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

PLflex
BBa_J176040 Version 1 (Component)
PLflex

RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

BBa_J176130
BBa_J176130 Version 1 (Component)
PLflex4

BBa_J176132
BBa_J176132 Version 1 (Component)
PLrigid4

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

RFC105 A
BBa_K1362414 Version 1 (Component)
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A

BBa_K2022003
BBa_K2022003 Version 1 (Component)
TolR, Periplasmic Domain, His-tag

BBa_J04031
BBa_J04031 Version 1 (Component)
GFP w/LVA tag

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

BBa_K1462503
BBa_K1462503 Version 1 (Component)
SH3 domain + Linker

BBa_K2022002
BBa_K2022002 Version 1 (Component)
TolR, Periplasmic Domain

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

HA
BBa_I712008 Version 1 (Component)
HA affinity tag

PelB
BBa_J32015 Version 1 (Component)
PelB leader sequence; directs protein to E. coli periplasmic membrane

NLS
BBa_J176008 Version 1 (Component)
NLS

BBa_K165006
BBa_K165006 Version 1 (Component)
Zif268-HIV DNA-binding domain

BBa_K300002
BBa_K300002 Version 1 (Component)
Phasin (PhaP) - head domain

BBa_K105012
BBa_K105012 Version 1 (Component)
10 aa flexible protein domain linker

PEST
BBa_J176018 Version 1 (Component)
PEST

Fok_a
BBa_K243000 Version 1 (Component)
Protein domain (active) of the restriction endonuclease FokI

linker
BBa_K133132 Version 1 (Component)
8 aa protein domain linker

RFC105 CC
BBa_K1362419 Version 1 (Component)
C-terminal splicing overhang CAAC=1/3?+Asn RFC[105] standard overhang CC

Gal4DB
BBa_J176020 Version 1 (Component)
Gal4 DB

BBa_J70594
BBa_J70594 Version 1 (Component)
RFC12 TAATAA Tail Domain

preSCsite
BBa_J18919 Version 1 (Component)
preScission protease cleavage site (E. coli-optimized)

BBa_K1462502
BBa_K1462502 Version 1 (Component)
PDZ domain + Linker

SPzim17
BBa_K809201 Version 1 (Component)
signal peptide of ZIM17

mCherry
BBa_K165004 Version 1 (Component)
mCherry, yeast optimized for fusion

BBa_K1321002
BBa_K1321002 Version 1 (Component)
Re-entry of BBa_K863111, Cellulose Binding Domain CBDclos in rfc25

NLSmycSTOP
BBa_J176031 Version 1 (Component)
NLS myc stop

BBa_K1150016
BBa_K1150016 Version 1 (Component)
HA tag

BBa_K1223006
BBa_K1223006 Version 1 (Component)
His-Tag

BBa_K1321003
BBa_K1321003 Version 1 (Component)
Re-entry of BBa_K863101, Cellulose Binding Domain CBDcex in rfc25

NpuDnaE(N)
BBa_K1362400 Version 1 (Component)
NpuDnaE N-Intein cloning piece

BBa_K554009
BBa_K554009 Version 1 (Component)
TolC

BBa_K2022008
BBa_K2022008 Version 1 (Component)
T7 Expression Construct for tolR, Periplasmic Domain

BBa_K1423000
BBa_K1423000 Version 1 (Component)
BCLA N-terminal Domain