Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
BBa_B0034BBa_B0034 Version 1 (Component)RBS (Elowitz 1999) -- defines RBS efficiency
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
BBa_B0030BBa_B0030 Version 1 (Component)RBS.1 (strong) -- modified from R. Weiss
BBa_B0032BBa_B0032 Version 1 (Component)RBS.3 (medium) -- derivative of BBa_0030
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
Scar25 ATGBBa_K1319106 Version 1 (Component)RFC 25 Translation Start Scar Sequence
BBa_J04500BBa_J04500 Version 1 (Component)IPTG inducible promoter with RBS
BBa_B0033BBa_B0033 Version 1 (Component)RBS.4 (weaker) -- derivative of BBa_0030
BBa_K1758100BBa_K1758100 Version 1 (Component)Translation enhancing 5-UTR containing g10-L RBS
BBa_K390018BBa_K390018 Version 1 (Component)sigA promoter, 5'UTR, and RBS
pT7BBa_K525998 Version 1 (Component)Promoter T7 and RBS
CouplingCoupling Version 1 (Component)Translational coupler
BBa_K390017BBa_K390017 Version 1 (Component)psbA2 promoter, 5'UTR, and RBS
T7 RBSBBa_K1362090 Version 1 (Component)strong T7 RBS
RBS-RFPBBa_K093005 Version 1 (Component)RFP with RBS
BBa_K1758106BBa_K1758106 Version 1 (Component)Translation enhancing 5-UTR + mRFP under control of T7 promoter
BBa_K1758101BBa_K1758101 Version 1 (Component)Translation enhancing 5-UTR + sfGFP
BBa_C0261BBa_C0261 Version 1 (Component)AHL-making Enzyme, luxI (+RBS)
BBa_M36009BBa_M36009 Version 1 (Component)5' Bicistronic UTR (strong), does not include ATG start
BBa_K914003BBa_K914003 Version 1 (Component)L-rhamnose-inducible promoter (pRha)
//rbsrbs Version 1 (Collection) RBS-spoVGBBa_K143021 Version 1 (Component)SpoVG ribosome binding site (RBS) for B. subtilis
BBa_J44001BBa_J44001 Version 1 (Component)Reverse RBS (RBS<sub>rev</sub>) -- corresponds to BBa_B0030
BBa_K390001BBa_K390001 Version 1 (Component)5'UTR and native RBS from psbA2 in Synechocystis sp. PCC 6803
BBa_K390002BBa_K390002 Version 1 (Component)5'UTR and consensus RBS from psbA2 in Synechocystis sp. PCC 6803
BBa_E0430BBa_E0430 Version 1 (Component)EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)
RBS_cassetteRBS_cassette Version 1 (Component)AvrII restriction site (cctagg), overlapped with RBS (aggagga)
BBa_K1758102BBa_K1758102 Version 1 (Component)Translation enhancing 5-UTR + sfGFP under control of T7 promoter
BBa_M50008BBa_M50008 Version 1 (Component)U10 Thermosensitive 5'-UTR (includes RBS)
eyfpBBa_E0031 Version 1 (Component)EYFP (RBS+ LVA-)
BBa_K165002BBa_K165002 Version 1 (Component)Kozak sequence (yeast RBS)
PBS_F24PBS_F24 Version 1 (Component) BBa_P10000BBa_P10000 Version 1 (Component)LexA promoter with 5' UTR
BBa_K390016BBa_K390016 Version 1 (Component)psaAB operon promoter, 5'UTR, and RBS
BBa_I9026BBa_I9026 Version 1 (Component)rhlI translational unit
BBa_K530019BBa_K530019 Version 1 (Component)HHO1 Yeast UTR
ChiPBBa_K564004 Version 1 (Component)ChiP promoter and 5' UTR; WT RBS (AAGAGG)
ecfpBBa_E0021 Version 1 (Component)ECFP (RBS+ LVA-)
BBa_M36001BBa_M36001 Version 1 (Component)5' Bicistronic UTR (medium), does not include ATG start
BBa_K390015BBa_K390015 Version 1 (Component)petBD operon promoter, 5'UTR, and RBS
ChiPmBBa_K564015 Version 1 (Component)ChiP promoter and 5' UTR; RBS mutated AAGAGG-->AGGAGA
BBa_M36282BBa_M36282 Version 1 (Component)5' Bicistronic UTR, includes ATG start codon
RBS-GFP-TeBBa_I763020 Version 1 (Component)RBS-GFP (+LVA Tag)-Terminators