Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

iGEM Feature Libraries
igem_feature_libraries_collection Version 1 (Unknown)
A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Free Genes Feature Libraries
free_genes_feature_libraries_collection Version 1 (Unknown)
A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_J04450
BBa_J04450 Version 1 (Component)
RFP Coding Device

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

BBa_J04031
BBa_J04031 Version 1 (Component)
GFP w/LVA tag

RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop

CD4 signal
BBa_I712009 Version 1 (Component)
CD4 signal peptide; localizes to plasma membrane

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

HA
BBa_I712008 Version 1 (Component)
HA affinity tag

NLS
BBa_J176008 Version 1 (Component)
NLS

BBa_K105012
BBa_K105012 Version 1 (Component)
10 aa flexible protein domain linker

PEST
BBa_J176018 Version 1 (Component)
PEST

linker
BBa_K133132 Version 1 (Component)
8 aa protein domain linker

BBa_K1989039
BBa_K1989039 Version 1 (Component)
Triplespytag with mRFP, His-tag , FlAsH-tag and signal peptide PelB

PelB
BBa_J32015 Version 1 (Component)
PelB leader sequence; directs protein to E. coli periplasmic membrane

BBa_J04051
BBa_J04051 Version 1 (Component)
RFP with tag

BBa_K1989048
BBa_K1989048 Version 1 (Component)
Triplespytag with amilGFP, His-tag , FlAsH-tag and signal peptide PelB

NLSmycSTOP
BBa_J176031 Version 1 (Component)
NLS myc stop

BBa_K1150016
BBa_K1150016 Version 1 (Component)
HA tag

BBa_K1989037
BBa_K1989037 Version 1 (Component)
Triplespytag with mRFP, His-tag , FlAsH-tag and signal peptide PhoA

BBa_K1989038
BBa_K1989038 Version 1 (Component)
Triplespytag with mRFP, His-tag , FlAsH-tag and signal peptide LTIIB

BBa_K1989036
BBa_K1989036 Version 1 (Component)
Triplespytag with mRFP, His-tag , FlAsH-tag and signal peptide OmpA

mCherry
BBa_J06504 Version 1 (Component)
monomeric RFP optimized for bacteria

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

BBa_K1989044
BBa_K1989044 Version 1 (Component)
Triplespytag with mRFP, FlAsH-tag and signal peptide LipA

BBa_K1989042
BBa_K1989042 Version 1 (Component)
Triplespytag with mRFP, FlAsH-tag and signal peptide SacB

BBa_K1989040
BBa_K1989040 Version 1 (Component)
Triplespytag with mRFP, FlAsH-tag and signal peptide ImdA

BBa_K1989043
BBa_K1989043 Version 1 (Component)
Triplespytag with mRFP, FlAsH-tag and signal peptide YjfA

BBa_K1989041
BBa_K1989041 Version 1 (Component)
Triplespytag with mRFP, FlAsH-tag and signal peptide NprE

BBa_K1989020
BBa_K1989020 Version 1 (Component)
Triplespycatcher with His-tag , FlAsH-tag and signal peptide PelB

BBa_K1989046
BBa_K1989046 Version 1 (Component)
Triplespytag with amilGFP, His-tag , FlAsH-tag and signal peptide PhoA

BBa_K1989047
BBa_K1989047 Version 1 (Component)
Triplespytag with amilGFP, His-tag , FlAsH-tag and signal peptide LTIIB

BBa_K1989051
BBa_K1989051 Version 1 (Component)
Triplespytag with eforRed, His-tag , FlAsH-tag and signal peptide LTIIB

BBa_K1989045
BBa_K1989045 Version 1 (Component)
Triplespytag with amilGFP, His-tag , FlAsH-tag and signal peptide OmpA

BBa_K1989049
BBa_K1989049 Version 1 (Component)
Triplespytag with eforRed, His-tag , FlAsH-tag and signal peptide OmpA

BBa_K1989050
BBa_K1989050 Version 1 (Component)
Triplespytag with eforRed, His-tag , FlAsH-tag and signal peptide PhoA

RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

BBa_K1989030
BBa_K1989030 Version 1 (Component)
Triple spytag with mSA, His-tag , FlAsH-tag and signal peptide PelB