Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

luxr
BBa_C0062 Version 1 (Component)
luxR repressor/activator, (no LVA?)

GFP report
BBa_E0240 Version 1 (Component)
GFP generator

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_M36983
BBa_M36983 Version 1 (Component)
Type II Secreted Antifreeze Actuator in E. coli (5-Repeat) Correct

BBa_M36982
BBa_M36982 Version 1 (Component)
Type II Secreted Antifreeze Actuator in E. coli (1-Repeat) Correct

BBa_M36990
BBa_M36990 Version 1 (Component)
Type II Secreted Antifreeze Actuator in E. coli (5-Repeat)

BBa_M36995
BBa_M36995 Version 1 (Component)
Type II Secreted Antifreeze Actuator in E. coli (5-Repeat)

BBa_M36994
BBa_M36994 Version 1 (Component)
Type II Secreted Antifreeze Actuator in E. coli (1-Repeat)

BBa_M36991
BBa_M36991 Version 1 (Component)
Type II Secreted Antifreeze Actuator in E. coli (1-Repeat)

BBa_M36993
BBa_M36993 Version 1 (Component)
Type II Secreted Antifreeze Actuator in E. coli (10-Repeat)

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

BBa_M36992
BBa_M36992 Version 1 (Component)
Type II Secreted Antifreeze Actuator in E.coli (10-Repeat)

BBa_J70029
BBa_J70029 Version 1 (Component)
RBS + mCherry gene, optimized for expression in Me. florum and E. coli

PelB
BBa_J32015 Version 1 (Component)
PelB leader sequence; directs protein to E. coli periplasmic membrane

lasR
BBa_C0179 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(no LVA)

const.
BBa_R1075 Version 1 (Component)
Constitutive Promoter II

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

sacB
BBa_K322921 Version 1 (Component)
B. subtilis levansucrase. Lethal to E. coli in presence of sucrose.

BBa_M36981
BBa_M36981 Version 1 (Component)
Type II Signal Peptide (Correct)

BBa_K731722
BBa_K731722 Version 1 (Component)
T1 terminator from E. coli rrnB

BBa_J33201
BBa_J33201 Version 1 (Component)
E. coli chromosomal ars promoter with arsR repressor gene

rhlR
BBa_C0071 Version 1 (Component)
rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)

BBa_I746352
BBa_I746352 Version 1 (Component)
delta activator from phiR73 phage

cinr
BBa_C0077 Version 1 (Component)
cinR activator from Rhizobium leguminosarum (+LVA)

preSCsite
BBa_J18919 Version 1 (Component)
preScission protease cleavage site (E. coli-optimized)

BBa_I746200
BBa_I746200 Version 1 (Component)
FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.

BBa_M36726
BBa_M36726 Version 1 (Component)
Type II Signal Peptide

BBa_K608351
BBa_K608351 Version 1 (Component)
(correct) temperature sensitive promoter

rhIR
BBa_C0171 Version 1 (Component)
rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)

BBa_M36999
BBa_M36999 Version 1 (Component)
Non-Glycolated Antifreeze Protein (AFP) - 5-Repeated Segment

adhB
BBa_K173017 Version 1 (Component)
alcohol dehydrogenase II from Z. mobilis (codon optimized for E. coli)

BBa_E0044
BBa_E0044 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)

BBa_K912001
BBa_K912001 Version 1 (Component)
E. coli phytase (appa phytase)

BBa_K1445001
BBa_K1445001 Version 1 (Component)
Endogenous Type II CRISPR-Cas9 phagemid

MccS
BBa_K1659100 Version 1 (Component)
Microcin S, a Class II microcin produced by probiotic E. coli G3/10

TEVsite
BBa_J18918 Version 1 (Component)
TEV cleavage site (E. coli)

BBa_I746350
BBa_I746350 Version 1 (Component)
ogr activator from P2 phage

BBa_K953004
BBa_K953004 Version 1 (Component)
Hemeoxygenase with RBS (E. coli Codon Optimised)

BBa_K1172901
BBa_K1172901 Version 1 (Component)
Alanine racemase from ''E. coli''