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Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Public

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Public

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

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BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

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BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

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tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

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Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

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BBa_J04450
BBa_J04450 Version 1 (Component)
RFP Coding Device

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cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

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GFP report
BBa_E0240 Version 1 (Component)
GFP generator

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eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

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amilCP
BBa_K592009 Version 1 (Component)
amilCP, blue chromoprotein

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luxI
BBa_C0061 Version 1 (Component)
autoinducer synthetase for AHL

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BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

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BBa_E0430
BBa_E0430 Version 1 (Component)
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)

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ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

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BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

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BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

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BBa_K1357009
BBa_K1357009 Version 1 (Component)
amilCP Reporter (RBS+Coding+Term)

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BBa_E0422
BBa_E0422 Version 1 (Component)
ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)

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BBa_E0420
BBa_E0420 Version 1 (Component)
ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)

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BBa_I746916
BBa_I746916 Version 1 (Component)
superfolder GFP coding sequence

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pT7
BBa_K525998 Version 1 (Component)
Promoter T7 and RBS

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aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

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lacZ-alpha
BBa_I732006 Version 1 (Component)
lacZ alpha fragment

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rrnO
rrnO_ter Version (Component)

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BBa_I13401
BBa_I13401 Version 1 (Component)
GFP reporter for RHS of library test constructs

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BBa_I732820
BBa_I732820 Version 1 (Component)
LacI (RBS+, TERM+)

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T7 RBS
BBa_K1362090 Version 1 (Component)
strong T7 RBS

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BBa_E0432
BBa_E0432 Version 1 (Component)
EYFP (RBS+ LVA+ TERM) (B0034.E0032.B0015)

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RBS-RFP
BBa_K093005 Version 1 (Component)
RFP with RBS

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cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

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LacI
LacI Version 1 (Component)

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BBa_C0261
BBa_C0261 Version 1 (Component)
AHL-making Enzyme, luxI (+RBS)

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lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

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BBa_K118002
BBa_K118002 Version 1 (Component)
crtB coding sequence encoding phytoene synthase

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BBa_K118003
BBa_K118003 Version 1 (Component)
crtI coding sequence encoding phytoene dehydrogenase

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rrnO_ter_seq
rrnO_ter_seq Version (Sequence)

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//rbs
rbs Version 1 (Collection)

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BBa_K592025
BBa_K592025 Version 1 (Component)
B0034-amilCP

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BBa_K516022
BBa_K516022 Version 1 (Component)
AiiA protein generator TERM+ RBS+(B0032)

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RBS-spoVG
BBa_K143021 Version 1 (Component)
SpoVG ribosome binding site (RBS) for B. subtilis

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RBS_SpaR
RBS_SpaR Version (Component)

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RBS_SpaK
RBS_SpaK Version (Component)

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amilGFP
BBa_K592010 Version 1 (Component)
amilGFP, yellow chromoprotein

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araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

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c2 P22
BBa_C0053 Version 1 (Component)
c2 repressor from Salmonella phage P22 (+LVA)

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BBa_J44001
BBa_J44001 Version 1 (Component)
Reverse RBS (RBS<sub>rev</sub>) -- corresponds to BBa_B0030

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RBS_SpaR_seq
RBS_SpaR_seq Version (Sequence)

Public

RBS_SpaK_seq
RBS_SpaK_seq Version (Sequence)

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