NAT (Nourseothricin Resistance/NrsR)
Nourseothricin_resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

luxr
BBa_C0062 Version 1 (Component)
luxR repressor/activator, (no LVA?)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

BBa_K779131
BBa_K779131 Version 1 (Component)
e - In vitro NOT gate MammoBlock

BBa_K779128
BBa_K779128 Version 1 (Component)
c3 - In vitro NOT gate MammoBlock

BBa_K779123
BBa_K779123 Version 1 (Component)
a - In vitro NOT gate MammoBlock

BBa_K779130
BBa_K779130 Version 1 (Component)
d2 - In vitro NOT gate MammoBlock

BBa_K779124
BBa_K779124 Version 1 (Component)
b1 - In vitro NOT gate MammoBlock

BBa_K779129
BBa_K779129 Version 1 (Component)
d1 - In vitro NOT gate MammoBlock

BBa_K779125
BBa_K779125 Version 1 (Component)
b2 - In vitro NOT gate MammoBlock

BBa_K779126
BBa_K779126 Version 1 (Component)
c1 - In vitro NOT gate MammoBlock

BBa_K779127
BBa_K779127 Version 1 (Component)
c2 - In vitro NOT gate MammoBlock

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

luxI
BBa_C0161 Version 1 (Component)
autoinducer synthetase for AHL (no LVA)

BBa_K779200
BBa_K779200 Version 1 (Component)
Hef1A MammoBlock

BBa_J70029
BBa_J70029 Version 1 (Component)
RBS + mCherry gene, optimized for expression in Me. florum and E. coli

Pnot(O55)
BBa_I732205 Version 1 (Component)
NOT Gate Promoter Family Member (D001O55)

BBa_K415506
BBa_K415506 Version 1 (Component)
pTRE-Tight L4R1 MammoBlock

sacB
BBa_K322921 Version 1 (Component)
B. subtilis levansucrase. Lethal to E. coli in presence of sucrose.

Restriction_sites
Restriction_sites Version 1 (Component)
SacI - KpnI - SmaI - BamHI - XbaI - SalI - PstI - SphI - HindIII - NotI

lasR
BBa_C0179 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(no LVA)

BBa_K511003
BBa_K511003 Version 1 (Component)
UAS-Gal4 Promoter MammoBlock

BBa_K1051003
BBa_K1051003 Version 1 (Component)
Stop codon free GFP in RFC[23] standard

BBa_K415508
BBa_K415508 Version 1 (Component)
phEF1a L4R1 MammoBlock Entry Vector

PelB
BBa_J32015 Version 1 (Component)
PelB leader sequence; directs protein to E. coli periplasmic membrane

Pnot(O11)
BBa_I732201 Version 1 (Component)
NOT Gate Promoter Family Member (D001O11)

Pnot(O1wt1
BBa_I732200 Version 1 (Component)
NOT Gate Promoter Family Member (D001O1wt1)

BBa_M36009
BBa_M36009 Version 1 (Component)
5' Bicistronic UTR (strong), does not include ATG start

BBa_K1321002
BBa_K1321002 Version 1 (Component)
Re-entry of BBa_K863111, Cellulose Binding Domain CBDclos in rfc25

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

BBa_K1051000
BBa_K1051000 Version 1 (Component)
Stop codon free RFP in RFC[23] standard

BBa_K511102
BBa_K511102 Version 1 (Component)
mKate MammoBlock

BBa_K1321003
BBa_K1321003 Version 1 (Component)
Re-entry of BBa_K863101, Cellulose Binding Domain CBDcex in rfc25

Pnot(O33)
BBa_I732203 Version 1 (Component)
NOT Gate Promoter Family Member (D001O33)

Pnot(O66)
BBa_I732206 Version 1 (Component)
NOT Gate Promoter Family Member (D001O66)

Pnot(O44)
BBa_I732204 Version 1 (Component)
NOT Gate Promoter Family Member (D001O44)

Pnot(O22)
BBa_I732202 Version 1 (Component)
NOT Gate Promoter Family Member (D001O22)

Pnot(O77)
BBa_I732207 Version 1 (Component)
NOT Gate Promoter Family Member (D001O77)

BBa_K1363603
BBa_K1363603 Version 1 (Component)
gate of no of RNA logic gates

BBa_K731722
BBa_K731722 Version 1 (Component)
T1 terminator from E. coli rrnB

BBa_J33201
BBa_J33201 Version 1 (Component)
E. coli chromosomal ars promoter with arsR repressor gene