ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

lacI+pL
BBa_R0011 Version 1 (Component)
Promoter (lacI regulated, lambda pL hybrid)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

cI lam
BBa_R0051 Version 1 (Component)
promoter (lambda cI regulated)

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

c2 P22
BBa_C0053 Version 1 (Component)
c2 repressor from Salmonella phage P22 (+LVA)

BBa_I11021
BBa_I11021 Version 1 (Component)
excisionase from E. coli phage lambda (removes prophage from host genome)

BBa_I712074
BBa_I712074 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)

BBa_I11031
BBa_I11031 Version 1 (Component)
excisionase from E. coli phage P22 (removes prophage from host genome)

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

cI HK022
BBa_C0050 Version 1 (Component)
cI repressor from phage HK022 (+LVA?)

cI+luxR
BBa_R0065 Version 1 (Component)
Promoter (lambda cI and luxR regulated -- hybrid)

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

BBa_I746352
BBa_I746352 Version 1 (Component)
delta activator from phiR73 phage

BBa_K2066019
BBa_K2066019 Version 1 (Component)
UNS 3 Sequence, from Torella et al., 2013

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

BBa_I746350
BBa_I746350 Version 1 (Component)
ogr activator from P2 phage

BBa_I15008
BBa_I15008 Version 1 (Component)
heme oxygenase (ho1) from Synechocystis

BBa_I746361
BBa_I746361 Version 1 (Component)
PO promoter from P2 phage

BBa_I15009
BBa_I15009 Version 1 (Component)
phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis

tADH1
BBa_J63002 Version 1 (Component)
ADH1 terminator from S. cerevisiae

BBa_K731722
BBa_K731722 Version 1 (Component)
T1 terminator from E. coli rrnB

pRE
BBa_K116603 Version 1 (Component)
pRE promoter from λ phage

CII
BBa_K116602 Version 1 (Component)
CII coding region from λ phage

lasR
BBa_C0179 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(no LVA)

BBa_C0056
BBa_C0056 Version 1 (Component)
cI repressor from phage 434 (no LVA)

BBa_I712019
BBa_I712019 Version 1 (Component)
Firefly luciferase - luciferase from Photinus pyralis

EYFP
BBa_E0034 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_I11020
BBa_I11020 Version 1 (Component)
integrase from E. coli phage lambda