Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

p(tetR)
BBa_R0040 Version 1 (Component)
TetR repressible promoter

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

P(Lac) IQ
BBa_I14032 Version 1 (Component)
promoter P(Lac) IQ

luxI
BBa_C0061 Version 1 (Component)
autoinducer synthetase for AHL

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

cI lam
BBa_R0051 Version 1 (Component)
promoter (lambda cI regulated)

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

BBa_P0440
BBa_P0440 Version 1 (Component)
PoPS -> TetR [S0151]

tetR
BBa_Q04400 Version 1 (Component)
QPI (B0034.C0040.B0015.R0040)

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

LasR+PAI
BBa_R0079 Version 1 (Component)
Promoter (LasR & PAI regulated)

luxI
BBa_C0161 Version 1 (Component)
autoinducer synthetase for AHL (no LVA)

lasI
BBa_C0078 Version 1 (Component)
autoinducer synthetase for PAI from Pseudomonas aeruginosa

tetA(C)f
BBa_J31007 Version 1 (Component)
tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]

pLux/Tet
BBa_K176000 Version 1 (Component)
pLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS

BBa_J13002
BBa_J13002 Version 1 (Component)
TetR repressed POPS/RIPS generator

lasR
BBa_C0179 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(no LVA)

Las CIO
BBa_I14016 Version 1 (Component)
P(Las) CIO

las TetO
BBa_I14015 Version 1 (Component)
P(Las) TetO

rhII
BBa_C0070 Version 1 (Component)
autoinducer synthetase for N-butyryl-HSL (BHL) and HHL

BBa_K176028
BBa_K176028 Version 1 (Component)
TetR repressible AHL->PoPS Receiver (Very High LuxR): pCon 0.70->luxR+pLux/Tet->PoPS

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

cinI
BBa_C0076 Version 1 (Component)
autoinducer synthetase

BBa_J28029
BBa_J28029 Version 1 (Component)
las autoinducer, P(tet)/tetR control

Cat
BBa_I14033 Version 1 (Component)
P(Cat)

BBa_K624001
BBa_K624001 Version 1 (Component)
tetR+RBS+GFP+ter

tetA(C)b
BBa_J31006 Version 1 (Component)
tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]

BBa_J61002
BBa_J61002 Version 1 (Component)
EX-Ptet-S-rbsRFP-P "RFP reporter"

BBa_J28027
BBa_J28027 Version 1 (Component)
lux autoinducer, P(tet)/tetR control

BBa_K823003
BBa_K823003 Version 1 (Component)
P_veg

RhI
BBa_I14017 Version 1 (Component)
P(Rhl)

IBMc226
IBMc226 Version 1 (Component)
pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter

tetR
BBa_Q01400 Version 1 (Component)
QPI (TetR, Medium RBS)

aiiA
BBa_C0160 Version 1 (Component)
autoinducer inactivation enzyme aiiA (no LVA)