Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
BBa_B0010BBa_B0010 Version 1 (Component)T1 from E. coli rrnB
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_B0034BBa_B0034 Version 1 (Component)RBS (Elowitz 1999) -- defines RBS efficiency
p(tetR)BBa_R0040 Version 1 (Component)TetR repressible promoter
BBa_B0030BBa_B0030 Version 1 (Component)RBS.1 (strong) -- modified from R. Weiss
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
lacIBBa_C0012 Version 1 (Component)lacI repressor from E. coli (+LVA)
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
cI lamBBa_C0051 Version 1 (Component)cI repressor from E. coli phage lambda (+LVA)
BBa_B0032BBa_B0032 Version 1 (Component)RBS.3 (medium) -- derivative of BBa_0030
pBad/araCBBa_I0500 Version 1 (Component)Inducible pBad/araC promoter
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
P(Lac) IQBBa_I14032 Version 1 (Component)promoter P(Lac) IQ
BBa_I13453BBa_I13453 Version 1 (Component)Pbad promoter
pBADBBa_K206000 Version 1 (Component)pBAD strong
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
BBa_J15001BBa_J15001 Version 1 (Component)strong synthetic E. coli ribosome binding site with SacI site.
lasrBBa_C0079 Version 1 (Component)lasR activator from P. aeruginosa PAO1(+LVA)
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
Scar 25BBa_B0105 Version 1 (Component)RFC 25 Scar Sequence
lasRBBa_C0179 Version 1 (Component)lasR activator from P. aeruginosa PAO1(no LVA)
LacIBBa_R0010 Version 1 (Component)promoter (lacI regulated)
BBa_I712074BBa_I712074 Version 1 (Component)T7 promoter (strong promoter from T7 bacteriophage)
BBa_M36010BBa_M36010 Version 1 (Component)Transcription Terminator (Strong)
rhlRBBa_C0071 Version 1 (Component)rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)
BBa_J56012BBa_J56012 Version 1 (Component)Invertible sequence of dna includes Ptrc promoter
BBa_J04500BBa_J04500 Version 1 (Component)IPTG inducible promoter with RBS
rhIRBBa_C0171 Version 1 (Component)rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)
lasIBBa_C0078 Version 1 (Component)autoinducer synthetase for PAI from Pseudomonas aeruginosa
T7 RBSBBa_K1362090 Version 1 (Component)strong T7 RBS
araCBBa_C0080 Version 1 (Component)araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
BBa_K608002BBa_K608002 Version 1 (Component)strong Promoter and strong RBS
BBa_B0033BBa_B0033 Version 1 (Component)RBS.4 (weaker) -- derivative of BBa_0030
Scar25 ATGBBa_K1319106 Version 1 (Component)RFC 25 Translation Start Scar Sequence
BBa_K209402BBa_K209402 Version 1 (Component)AarI A-B part, signal sequence-FLAG-YFP [SSFYFP]
BBa_K880005BBa_K880005 Version 1 (Component)Strong promoter, strong RBS combination for high expression levels of proteins
tetRBBa_C0040 Version 1 (Component)tetracycline repressor from transposon Tn10 (+LVA)