Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

BBa_B0032
BBa_B0032 Version 1 (Component)
RBS.3 (medium) -- derivative of BBa_0030

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

cI lam
BBa_R0051 Version 1 (Component)
promoter (lambda cI regulated)

luxr
BBa_C0062 Version 1 (Component)
luxR repressor/activator, (no LVA?)

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

cI+luxR
BBa_R0065 Version 1 (Component)
Promoter (lambda cI and luxR regulated -- hybrid)

araC
BBa_C0080 Version 1 (Component)
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)

BBa_B0031
BBa_B0031 Version 1 (Component)
RBS.2 (weak) -- derivative of BBa_0030

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_K750104
BBa_K750104 Version 1 (Component)
pBADcIT-1.0:cI protein expression system activated by arabinose(RBS strength:1.0)

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_K750107
BBa_K750107 Version 1 (Component)
pBADcIT-0.07:cI protein expression system activated by arabinose(RBS strength:0.07)

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

BBa_K750106
BBa_K750106 Version 1 (Component)
pBADcIT-0.3:cI protein expression system activated by arabinose(RBS strength:0.3)

BBa_B0033
BBa_B0033 Version 1 (Component)
RBS.4 (weaker) -- derivative of BBa_0030

BBa_K750105
BBa_K750105 Version 1 (Component)
pBADcIT-0.6:cI protein expression system activated by arabinose(RBS strength:0.6)

BBa_K880005
BBa_K880005 Version 1 (Component)
Strong promoter, strong RBS combination for high expression levels of proteins

cI 434
BBa_R0052 Version 1 (Component)
Promoter (434 cI regulated)

Prm +
BBa_I12006 Version 1 (Component)
Modified lamdba Prm promoter (repressed by 434 cI)

lasr
BBa_C0079 Version 1 (Component)
lasR activator from P. aeruginosa PAO1(+LVA)

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

BBa_K750103
BBa_K750103 Version 1 (Component)
PcIGLT:GFP(lva) repression system activated by cI protein

cI lam
BBa_R1051 Version 1 (Component)
Promoter, Standard (lambda cI regulated)<br>

Ara
BBa_K808000 Version 1 (Component)
araC-Pbad - Arabinose inducible regulatory promoter/repressor unit

pT7
BBa_K525998 Version 1 (Component)
Promoter T7 and RBS

cI HK022
BBa_C0050 Version 1 (Component)
cI repressor from phage HK022 (+LVA?)

BBa_K750003
BBa_K750003 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.3 strength)

BBa_K750002
BBa_K750002 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.6 strength)

BBa_K314103
BBa_K314103 Version 1 (Component)
Lac induced expression cassette

T7 RBS
BBa_K1362090 Version 1 (Component)
strong T7 RBS