NAT (Nourseothricin Resistance/NrsR)
Nourseothricin_resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

pSB1C3
pSB1C3 Version 1 (Component)
High copy BioBrick assembly plasmid

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

pBad/araC
BBa_I0500 Version 1 (Component)
Inducible pBad/araC promoter

BBa_I13453
BBa_I13453 Version 1 (Component)
Pbad promoter

BBa_B1001
BBa_B1001 Version 1 (Component)
Terminator (artifical, small, %T~=90)

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

IBMc180
IBMc180 Version 1 (Component)
pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI

pBAD
BBa_K206000 Version 1 (Component)
pBAD strong

pSB1AT3
pSB1AT3 Version 1 (Component)
High copy BioBrick assembly plasmid

BBa_B1006
BBa_B1006 Version 1 (Component)
Terminator (artificial, large, %T~>90)

IBMc190
IBMc190 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-10aa-ECF20(102-193)-T

Restriction_sites
Restriction_sites Version 1 (Component)
SacI - KpnI - SmaI - BamHI - XbaI - SalI - PstI - SphI - HindIII - NotI

IBMc191
IBMc191 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-15aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-15aa-ECF20(102-193)-T

BBa_J15001
BBa_J15001 Version 1 (Component)
strong synthetic E. coli ribosome binding site with SacI site.

IBMc397
IBMc397 Version 1 (Component)
pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T

IBMc360
IBMc360 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry(1-192)-G-M86(1-100)-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(101-154)-S-mCherry(193-236)-H6-T

IBMc188
IBMc188 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-2aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-2aa-ECF20(102-193)-T

IBMc189
IBMc189 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-5aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-5aa-ECF20(102-193)-T

IBMc378
IBMc378 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-17)-PI-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(18-154)-S-mCherry*(193-236)-H6-T

IBMc379
IBMc379 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-22)-LG-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(23-154)-S-mCherry*(193-236)-H6-T

IBMc380
IBMc380 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-39)-LD-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(40-154)-S-mCherry*(193-236)-H6-T

pSB1A2
pSB1A2 Version 1 (Component)
pSB1A2 (Replaced by pSB1A3)

IBMc396
IBMc396 Version 1 (Component)
pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T

IBMc233
IBMc233 Version 1 (Component)
pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI

Cat
BBa_I14033 Version 1 (Component)
P(Cat)

IBMc335
IBMc335 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEY-e-NpuNcage-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-NpuCcage-CFNS-mCherry*(193-236)-H6-Ter

5xGS
BBa_J18922 Version 1 (Component)
10aa [GS]x linker

IBMc187
IBMc187 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-T-cat-PBAD(SapI-)-RiboJ-BCD22-ECF20(102-193)-T

IBMc395
IBMc395 Version 1 (Component)
pSB3K3-TT-PBAD(SapI-)-B30-His-FKBP-14aa-T7RNAP(181+)-T

pSB1AK3
pSB1AK3 Version 1 (Component)
High copy BioBrick assembly plasmid

IBMc050
IBMc050 Version 1 (Component)
pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI

IBMc394
IBMc394 Version 1 (Component)
pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-14aa-FRB-T

IBMc460
IBMc460 Version 1 (Component)
pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI

IBMc318
IBMc318 Version 1 (Component)
pSB1A3-BbsI-G-ER-LBD-S-SapI

pSB1A3
pSB1A3 Version 1 (Component)
High copy BioBrick assembly plasmid

pSB4A3
pSB4A3 Version 1 (Component)
pSB4A3 (Replaced by pSB4A5)

pSB1K3
pSB1K3 Version 1 (Component)
High copy BioBrick assembly plasmid

IBMc071
IBMc071 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T

IBMc052
IBMc052 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*-T

IBMc471
IBMc471 Version 1 (Component)
pSB1A3-PBAD(SapI-)-B30-mCherry*(1-173)-Ter

IBMc473
IBMc473 Version 1 (Component)
pSB1A3-PBAD(SapI-)-B30-mCherry*(1-185)-Ter

IBMc470
IBMc470 Version 1 (Component)
pSB1A3-PBAD(SapI-)-B30-mCherry*(1-156)-Ter

IBMc469
IBMc469 Version 1 (Component)
pSB1A3-PBAD(SapI-)-B30-mCherry*(1-121)-Ter

IBMc472
IBMc472 Version 1 (Component)
pSB1A3-PBAD(SapI-)-B30-mCherry*(1-176)-Ter

IBMc475
IBMc475 Version 1 (Component)
pSB1A3-PBAD(SapI-)-B30-mCherry*(1-192)-Ter

IBMc474
IBMc474 Version 1 (Component)
pSB1A3-PBAD(SapI-)-B30-mCherry*(1-191)-Ter