ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

BBa_B0030
BBa_B0030 Version 1 (Component)
RBS.1 (strong) -- modified from R. Weiss

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

tetR
BBa_C0040 Version 1 (Component)
tetracycline repressor from transposon Tn10 (+LVA)

eyfp
BBa_E0030 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

lacI
BBa_C0012 Version 1 (Component)
lacI repressor from E. coli (+LVA)

YFP
BBa_E0032 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

ecfp
BBa_E0020 Version 1 (Component)
engineered cyan fluorescent protein derived from A. victoria GFP

mRFP1
BBa_E1010 Version 1 (Component)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

cI lam
BBa_C0051 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA)

BBa_I712019
BBa_I712019 Version 1 (Component)
Firefly luciferase - luciferase from Photinus pyralis

ECFP
BBa_E0022 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

BBa_K325210
BBa_K325210 Version 1 (Component)
Red Firefly Luciferase and LRE<BR><i>L. Cruciata<BR>(E. coli optimised)</i>

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

BBa_K563045
BBa_K563045 Version 1 (Component)
pYE MSC+Orange Firefly Luciferase+CYC1 TT , derived from BBa_K325211

BBa_K352011
BBa_K352011 Version 1 (Component)
pCooM Promoter coupled with RBS+RFP+Double terminator

BBa_K325219
BBa_K325219 Version 1 (Component)
Red Firefly Luciferase and LRE (under pBAD)<BR><i>L. Cruciata<BR>(E. coli optimised)</i>

BBa_K325218
BBa_K325218 Version 1 (Component)
Orange Firefly Luciferase (under pBAD)<BR><i>L. Cruciata<BR>(E. coli optimised)</i>

EYFP
BBa_E0034 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

BBa_J52008
BBa_J52008 Version 1 (Component)
luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)

BBa_K325209
BBa_K325209 Version 1 (Component)
Green Firefly Luciferase and LRE (under pBAD)<BR><i>L. Cruciata<BR>(E. coli optimised)</i>

BBa_K325259
BBa_K325259 Version 1 (Component)
Yellow Firefly Luciferase (under pBAD)<BR><i>L. Cruciata<BR>(E. coli optimised)</i>

BBa_K563046
BBa_K563046 Version 1 (Component)
pYE MCS+EPCI Firefly Luciferase+CYC1 TT, derived from BBa_K325101

BBa_K325101
BBa_K325101 Version 1 (Component)
EPIC Firefly Luciferase <BR><i>Photinus Pyralis<BR>(E. coli optimised)</i>

BBa_I712074
BBa_I712074 Version 1 (Component)
T7 promoter (strong promoter from T7 bacteriophage)

BBa_K325211
BBa_K325211 Version 1 (Component)
Orange Firefly Luciferase<BR><i>L. Cruciata<BR>(E. coli optimised)</i>

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

BBa_K323216
BBa_K323216 Version 1 (Component)
T7 promoter

BBa_K323011
BBa_K323011 Version 1 (Component)
N-split fragment of mCerulean with stop

BBa_K323217
BBa_K323217 Version 1 (Component)
T7 promoter with start codon and hexahistidine tag

BBa_K322211
BBa_K322211 Version 1 (Component)
Red emitting firefly luciferase mutant (356K)

BBa_K1725211
BBa_K1725211 Version 1 (Component)
B0032 + LuxG

BBa_K323213
BBa_K323213 Version 1 (Component)
RBS crtO

BBa_K322115
BBa_K322115 Version 1 (Component)
phoA promoter

BBa_K322210
BBa_K322210 Version 1 (Component)
Chloramphenicol acetyltransferase (cat)

BBa_K2066018
BBa_K2066018 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

BBa_K329011
BBa_K329011 Version 1 (Component)
RBS + Xis-Int HK022

BBa_K322119
BBa_K322119 Version 1 (Component)
CcaS upstream + PhoR downstream

aiiA
BBa_C0060 Version 1 (Component)
autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

BBa_K654033
BBa_K654033 Version 1 (Component)
Firefly Luciferase + Terminator

BBa_K322117
BBa_K322117 Version 1 (Component)
phoA promoter and lacZ reporter

cI 434
BBa_C0052 Version 1 (Component)
cI repressor from phage 434 (+LVA)