BBa_K1938003
BBa_K1938003 Version 1 (Component)
pcrD

BBa_K2070012
BBa_K2070012 Version 1 (Component)
Pnrd promoter

BBa_K1938000
BBa_K1938000 Version 1 (Component)
pcrA

BBa_K1938001
BBa_K1938001 Version 1 (Component)
pcrB

BBa_K1938002
BBa_K1938002 Version 1 (Component)
pcrC

BBa_M36245
BBa_M36245 Version 1 (Component)
PCad Promoter

BBa_P101103
BBa_P101103 Version 1 (Component)
JMT RBS.ORF.Term (PCR)

BBa_C100003
BBa_C100003 Version 1 (Component)
ORF of the JMT odorant enzyme (PCR)

pyrD 3 IS
BBa_K143009 Version 1 (Component)
3??? Integration Sequence for the pyrD locus of B. subtilis

pyrD 5 IS
BBa_K143008 Version 1 (Component)
5??? Integration Sequence for the pyrD locus of B. subtilis

BBa_K146000
BBa_K146000 Version 1 (Component)
pCR2.1 PCR product cloning vector

BBa_P100103
BBa_P100103 Version 1 (Component)
JMT ORF (PCR) with RBS

BBa_P121103
BBa_P121103 Version 1 (Component)
JMT Expression Cassette (F2620, PCR)

BBa_P111103
BBa_P111103 Version 1 (Component)
JMT Expression Cassette (R0040,PCR)

BBa_K143007
BBa_K143007 Version 1 (Component)
5??? Integration Sequence for the PyrD locus of B.subtilis

BBa_K316020
BBa_K316020 Version 1 (Component)
B. subtilis genome integration vector, targets pyrD locus

BBa_K143003
BBa_K143003 Version 1 (Component)
5??? Integration Sequence for the PyrD locus of B. subtilis

Prefix-R
BBa_G1001 Version 1 (Component)
Reverse primer for amplifying BioBrick plasmid backbones by PCR (Prefix-r)

Suffix-F
BBa_G1000 Version 1 (Component)
Forward primer for amplifying BioBrick plasmid backbones by PCR (Suffix-f)

BBa_K1015002
BBa_K1015002 Version 1 (Component)
streptmycin resisitance gene (can PCR ampicillin resistance gene primer)

Prefix-R
BBa_G1003 Version 1 (Component)
Reverse primer for amplifying BioBrick plasmid backbones by PCR (Prefix-r)

Suffix-F
BBa_G1002 Version 1 (Component)
Forward primer for amplifying BioBrick plasmid backbones by PCR (Suffix-f)

BBa_K391004
BBa_K391004 Version 1 (Component)
Forward primer to PCR S. aureus plasmid replicon

BBa_K391005
BBa_K391005 Version 1 (Component)
Reverse primer to PCR S. aureus plasmid replicon

BBa_G0007
BBa_G0007 Version 1 (Component)
Short sequence to promoter addition of 3' A overhang during PCR

BBa_G0008
BBa_G0008 Version 1 (Component)
Short sequence to promoter addition of 3' A overhang during PCR

BBa_K391002
BBa_K391002 Version 1 (Component)
Forward Primer to PCR agrCA (with RBS) from S. aureus

BBa_K391003
BBa_K391003 Version 1 (Component)
Reverse Primer to PCR agrCA (with RBS) from S. aureus

BBa_J70342
BBa_J70342 Version 1 (Component)
J70315psri.f.1: J70315 psri forward part for pcr of S03621 (single stranded)

BBa_J70343
BBa_J70343 Version 1 (Component)
J70315psri.r.1: J70315 psri reverse part for pcr of S03621 (single stranded)

BBa_K1124123
BBa_K1124123 Version 1 (Component)
inverse PCR template for creating new sRNA (plambda-micC sRNA scaffold-terminator)

BBa_K737000
BBa_K737000 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.