ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
BBa_J85801BBa_J85801 Version 1 (Component)rtTa3-2A-NLS-mKate2
BBa_J85901BBa_J85901 Version 1 (Component)Hef1a-B1-rtTA3-2A-NLS-mKate2
NLSBBa_J176008 Version 1 (Component)NLS
NLSmycSTOPBBa_J176031 Version 1 (Component)NLS myc stop
NLS+TermBBa_K165018 Version 1 (Component)SV40 nuclear localization sequence + ADH1 transcription terminator
GC Optimised Sequence PullGC_Optimised Version 1 (Activity)Used to indicate that a sequence was GC optimised after pulling from the source and before submission
BBa_K2050421BBa_K2050421 Version 1 (Component)Thosea Asigna Virus 2A peptide linker (T2A)
BBa_K779312BBa_K779312 Version 1 (Component)mKate-HH - Hammerhead attached behind mKate MammoBlock
BBa_I712022BBa_I712022 Version 1 (Component)T7 RNA polymerase with NLS
BBa_K511102BBa_K511102 Version 1 (Component)mKate MammoBlock
BBa_K511203BBa_K511203 Version 1 (Component)rtTA3 Tetracycline-Inducible Transactivator MammoBlock
BBa_I712069BBa_I712069 Version 1 (Component)RBS-T7 RNA polymerase with NLS
BBa_K511911BBa_K511911 Version 1 (Component)Inducible rtTA Transactivator Generator (TRE-Tight-rtTA3) MammoBlock Device
BBa_K779305BBa_K779305 Version 1 (Component)rtTA3 MammoBlock
BBa_K779311BBa_K779311 Version 1 (Component)HH-mKate - Hammerhead attached to front of mKate MammoBlock
BBa_K1316000BBa_K1316000 Version 1 (Component)mKate2 protein
TNY2AKUBBBa_K076000 Version 1 (Component)pLV-TRE-Ngn1-linker-EYFP-2A-mKate-Ubc-Bla
BBa_K929001BBa_K929001 Version 1 (Component)AID without NES, with NLS and Kozak sequence
BBa_K511902BBa_K511902 Version 1 (Component)rtTA3 Transactivator Generator (Hef1a-rtTA3) MammoBlock Device
BBa_K1055000BBa_K1055000 Version 1 (Component)mKate is a fluorophor derived from dsRed
BBa_K1362057BBa_K1362057 Version 1 (Component)NLS as RFC[105] N-terminal insert
BBa_K1179022BBa_K1179022 Version 1 (Component)CAG_Acyl-TyA-rtTA3
ATA-rtTA3BBa_K1179004 Version 1 (Component)(Acyl-TyA-rtTA3) A membrane/exosome targeting rtTA3 fusion to send through exosomes
BBa_K511914BBa_K511914 Version 1 (Component)Inducible rtTA3 Transactivator Generator (UAS-Gal4-rtTA3) MammoBlock Device
BBa_K2040122BBa_K2040122 Version 1 (Component)KillerRed + NLS
BBa_K1316001BBa_K1316001 Version 1 (Component)mKate2 protein with double transcription terminator
BBa_K1965022BBa_K1965022 Version 1 (Component)nLuc:AP4:TEVs:P3mS-2A