BBa_K1014014
BBa_K1014014 Version 1 (Component)
hrpL AND Gate device

BBa_K779601
BBa_K779601 Version 1 (Component)
Hef1a-mKate MammoBlock Device

BBa_K1956023
BBa_K1956023 Version 1 (Component)
Noise in Device measurement

BBa_K779604
BBa_K779604 Version 1 (Component)
Hef1a-rTTA3 MammoBlock Device

BBa_K554016
BBa_K554016 Version 1 (Component)
flhDC GFP HlyA device

BBa_K554012
BBa_K554012 Version 1 (Component)
SoxS GFP HlyA device

BBa_K1420000
BBa_K1420000 Version 1 (Component)
Mer operon, biological system found to detoxify organic and inorganic forms of mercury

BBa_K1438030
BBa_K1438030 Version 1 (Component)
pQE_80L Expression Plasmid

BBa_K528019
BBa_K528019 Version 1 (Component)
Expression Adapter s05_12k

BBa_J72190
BBa_J72190 Version 1 (Component)
neuS expression cassette

BBa_K528015
BBa_K528015 Version 1 (Component)
Expression Adapter s00_45k

BBa_K2141000
BBa_K2141000 Version 1 (Component)
pET28a(+) expression plasmid

BBa_K528018
BBa_K528018 Version 1 (Component)
Expression Adapter s06_16k

BBa_J72194
BBa_J72194 Version 1 (Component)
neuS expression cassette

BBa_J72185
BBa_J72185 Version 1 (Component)
GFP expression cassette

BBa_J72182
BBa_J72182 Version 1 (Component)
repA expression cassette

BBa_K528020
BBa_K528020 Version 1 (Component)
Expression Adapter s03_65k

BBa_J72184
BBa_J72184 Version 1 (Component)
RepA Expression Cassette

BBa_J72193
BBa_J72193 Version 1 (Component)
manB expression cassette

BBa_K528017
BBa_K528017 Version 1 (Component)
Expression Adapter s02_20k

BBa_J72183
BBa_J72183 Version 1 (Component)
GFP Expression cassette

BBa_K528016
BBa_K528016 Version 1 (Component)
Expression Adapter s01_24k

BBa_J72191
BBa_J72191 Version 1 (Component)
wbbL expression cassette

BBa_M1780
BBa_M1780 Version 1 (Component)
IL-18 Gene

arsB
BBa_M36221 Version 1 (Component)
Ars B gene

BBa_K1780011
BBa_K1780011 Version 1 (Component)
c1 reppresor gene

BBa_M1781
BBa_M1781 Version 1 (Component)
Caspase 1 Gene

BBa_M36923
BBa_M36923 Version 1 (Component)
Ferredoxin producing gene

BBa_K1073035
BBa_K1073035 Version 1 (Component)
Inducible ampR expression combined with RhlR, LasI and eforRed expression cassettes

BBa_K823043
BBa_K823043 Version 1 (Component)
alternative sigma factor ecf41

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K121010
BBa_K121010 Version 1 (Component)
Ptet GFP on pSB6

BBa_I2029
BBa_I2029 Version 1 (Component)
RhlI/RhlR Signal Test (I2027.I2028)

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

BBa_K1654011
BBa_K1654011 Version 1 (Component)
LuxR (vibrio harveyi ) repressible expression of Naly

BBa_J49005
BBa_J49005 Version 1 (Component)
English Tea-time Gene

BBa_K1179015
BBa_K1179015 Version 1 (Component)
A U6_gRNA(Cr9) plasmid which produces Cr9 targeting guide RNA's for Cas9

BBa_K363006
BBa_K363006 Version 1 (Component)
A characterized calcium dependent response element binding site for the Crz1 activator

BBa_K1956044
BBa_K1956044 Version 1 (Component)
gRNA for mCherry gene

BBa_K1956042
BBa_K1956042 Version 1 (Component)
gRNA for Chloramphenicol gene

BBa_Z0118
BBa_Z0118 Version 1 (Component)
T7 gene 3

BBa_Z0153
BBa_Z0153 Version 1 (Component)
T7.2 gene 3

BBa_K089001
BBa_K089001 Version 1 (Component)
phaA gene

BBa_K089002
BBa_K089002 Version 1 (Component)
phaB gene

BBa_K341451
BBa_K341451 Version 1 (Component)
gam gene

BBa_K341450
BBa_K341450 Version 1 (Component)
beta gene

BBa_K341449
BBa_K341449 Version 1 (Component)
exo gene

BBa_K524004
BBa_K524004 Version 1 (Component)
pir gene

BBa_M11207
BBa_M11207 Version 1 (Component)
Device containing HXT7/mNarK promoters and GFP/RFP to monitor glucose/oxygen levels in S. cerevisiae

BBa_M11208
BBa_M11208 Version 1 (Component)
Device containing HXT1/mNarK promoters and GFP/RFP to monitor glucose/oxygen levels in S. cerevisiae