ssTorA_CS-
BBa_K627013 Version 1 (Component)
Fusion of ssTorA, cleavage site of 14_3C protease and b-lactamase

Si4-CBPa-B
BBa_K2053002 Version 1 (Component)
Fusion Si4-cellulose-binding domain-B domain protein A

cheZ Unit
BBa_K518007 Version 1 (Component)
cheZ expression cassette (no promoter)

BBa_K669006
BBa_K669006 Version 1 (Component)
Allows the measurement of the CBP promoter activity

BBa_K1631003
BBa_K1631003 Version 1 (Component)
Tlanslational unit of Colicin Lysis Protein (for colicin-E3)

BBa_I751410
BBa_I751410 Version 1 (Component)
BioBrick to Tokyo Standard adapter, for SpeI, forward

BBa_I751400
BBa_I751400 Version 1 (Component)
BioBrick to Tokyo Standard adapter, for XbaI, forward

R37
BBa_K780002 Version 1 (Component)
Strong RBS for Bacillus Subtilis

BBa_K199191
BBa_K199191 Version 1 (Component)
pLac+tRNA CCACC+pLac+tRNA AGGAC+pLac+tRNA CUACC

BBa_K531006
BBa_K531006 Version 1 (Component)
Optimized esp gene for <i>C. crescentus</i>

Venus C
BBa_K537006 Version 1 (Component)
Venus C-Fusion

BBa_K1127002
BBa_K1127002 Version 1 (Component)
IM9 and A3-Flg fusion for gold binding

BBa_K1033250
BBa_K1033250 Version 1 (Component)
mCherry fusion protein codon optimised for Lactobacillus reuteri

BBa_K189001
BBa_K189001 Version 1 (Component)
Nu1 and A of lambda genome

BBa_K1828002
BBa_K1828002 Version 1 (Component)
sgRNA for VicK under nlmAB promoter

BBa_K1465213
BBa_K1465213 Version 1 (Component)
RubisCO of Halothiobacillus neapolitanus under control of the ptac promoter

BBa_K1363603
BBa_K1363603 Version 1 (Component)
gate of no of RNA logic gates

BBa_K1363605
BBa_K1363605 Version 1 (Component)
gate of and of RNA logic gates

BBa_K1363602
BBa_K1363602 Version 1 (Component)
key of yes of RNA logic gates

BBa_K2184004
BBa_K2184004 Version 1 (Component)
Guid RNA for umami&monosodiom glutamat flavor

SbpA|LigA
BBa_K525006 Version 1 (Component)
Fusion Protein of S-Layer SbpA and LigA

BBa_K1778003
BBa_K1778003 Version 1 (Component)
RtTA-flag is a regulatory protein that can regulate the binding of tetracycline analogues.

BBa_J70662
BBa_J70662 Version 1 (Component)
Short fusion insert test, designed from J70590 to recreate J04430

BBa_K1707028
BBa_K1707028 Version 1 (Component)
CFP-ssRA downstream of a cI regulated promoter

BBa_K1187000
BBa_K1187000 Version 1 (Component)
Human insulin gene, optimized for expression in Pichia pastoris

BBa_Y00073
BBa_Y00073 Version 1 (Component)
S. pombe gene SPCC126.04c, coded for S. cerevisiae expression

BBa_K1159308
BBa_K1159308 Version 1 (Component)
Neomycin phosphotransferase II (nptII) plant expression cassette for G418 resistance

SbpA | YFP
BBa_K525405 Version 1 (Component)
Fusion Protein of S-Layer SbpA and mCitrine

SgsE | Red
BBa_K525312 Version 1 (Component)
Fusion Protein of S-Layer SgsE and Oxidoreductase

BBa_K112316
BBa_K112316 Version 1 (Component)
{a~lambda antiholin!}; adheres to Berkeley standard

BBa_K112310
BBa_K112310 Version 1 (Component)
{a~lambda holin!}; adheres to Berkeley standard

BBa_K284017
BBa_K284017 Version 1 (Component)
Lysozyme expression in response to lactate (DLD promoter)

BBa_K284015
BBa_K284015 Version 1 (Component)
Lysozyme expression in response to lactate (JEN1 promoter)

BBa_K1420000
BBa_K1420000 Version 1 (Component)
Mer operon, biological system found to detoxify organic and inorganic forms of mercury

BBa_K1124114
BBa_K1124114 Version 1 (Component)
anti-luxI sRNA (+termintor) (sRNA generator)

BBa_K750001
BBa_K750001 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 1.0 strength)

BBa_K1172908
BBa_K1172908 Version 1 (Component)
Part 1 of the Biosafety-System Lac of Growth (pRha lacI alr)

BBa_K1696017
BBa_K1696017 Version 1 (Component)
tag3 for mf-lon to recognize

BBa_K1696019
BBa_K1696019 Version 1 (Component)
tag5 for mf-lon to recognize

BBa_K1696020
BBa_K1696020 Version 1 (Component)
tag6 for mf-lon to recognize

BBa_K1696018
BBa_K1696018 Version 1 (Component)
tag4 for mf-lon to recognize

BBa_K855003
BBa_K855003 Version 1 (Component)
coding sequence for pvdQ with histag added to the C-terminal followed by stop codon

BBa_K1323000
BBa_K1323000 Version 1 (Component)
sgRNA (1): CRISPR Silencing of YFP

BBa_K1323001
BBa_K1323001 Version 1 (Component)
sgRNA (2): CRISPR Silencing of YFP

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K2057003
BBa_K2057003 Version 1 (Component)
LasR under the regulation of the promoter chiA74

BBa_K1381014
BBa_K1381014 Version 1 (Component)
Silencing sRNA (10 bases long) for USP45 (Based on Spot42)

BBa_K1381016
BBa_K1381016 Version 1 (Component)
Silencing sRNA (20 bases long) for USP45 (Based on Spot42)

BBa_K1381015
BBa_K1381015 Version 1 (Component)
Silencing sRNA (15 bases long) for USP45 (Based on Spot42)

BBa_K1407026
BBa_K1407026 Version 1 (Component)
detect the intensity of precA