BBa_J100269
BBa_J100269 Version 1 (Component)
SP-T7Promiscuous_L1-192.2 (Carlson et al. 2014)

BBa_J100270
BBa_J100270 Version 1 (Component)
SP-T7Specific_L2-48.3 (Carlson et al. 2014)

BBa_J119397
BBa_J119397 Version 1 (Component)
pJC173b gIII neg (Carlson et al. 2014)

BBa_J100263
BBa_J100263 Version 1 (Component)
RS10 (Wachsmuth et al. 2013) in J119361

BBa_J100266
BBa_J100266 Version 1 (Component)
pJC173g-SD8_AP-neg (Carlson et al. 2014)

BBa_K180006
BBa_K180006 Version 1 (Component)
Game of Life - Primary plasmid (part 2) [lux pR, GFP and LacI generator]

Digitalizer
Digitalizer_collection Version 1 (Collection)
A genetic device to digitalize gene expression into a sharp on/off signal.

BBa_J55000
BBa_J55000 Version 1 (Component)
Produce GFP in presence of AHL

BBa_I733005
BBa_I733005 Version 1 (Component)
Produce GFP in presence of AHL

BBa_K092900
BBa_K092900 Version 1 (Component)
RhlR controlled by a constitutive promoter, and LacI modified with a Rhl R promoter

BBa_K228013
BBa_K228013 Version 1 (Component)
(pSal PO) OR Gate - GFP

BBa_I733004
BBa_I733004 Version 1 (Component)
Produce LacZ alpha in response to AHL

BBa_K1778002
BBa_K1778002 Version 1 (Component)
TRE-CYC1TATA is a recombinant promoter, which is constructed in order to make the Tet-on system func

BBa_K2116006
BBa_K2116006 Version 1 (Component)
AND gate regulated by norR and esaR (three esaboxes)

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o

BBa_K315005
BBa_K315005 Version 1 (Component)
variant reverse lox site with 3 base changes in the spacer region (loxm2 reverse)

loxBri (R)
BBa_K315006 Version 1 (Component)
LoxBri (R)variant reverse lox site with 3 base changes in the spacer region

SUPPORT DE
BBa_K1433022 Version 1 (Component)
rT-rKan-rRBS-attB-P-attP-RBS-lambda red-RBS-Chl-T-P-RBS-gp47-tag-T

BBa_K1341011
BBa_K1341011 Version 1 (Component)
OR LOGIC GATE IN Graph Theory (GFP OUTPUT DEVICE)

BBa_J04795
BBa_J04795 Version 1 (Component)
Riboswitch designed to turn "ON" a protein

PchiP-lacZ
BBa_K564002 Version 1 (Component)
Chitoporin fused with lacZ - target for sRNA based regulation

BBa_I763003
BBa_I763003 Version 1 (Component)
GFP coding device switched on by IPTG

BBa_K1363604
BBa_K1363604 Version 1 (Component)
key of no of RNA logic gates

BBa_J119300
BBa_J119300 Version 1 (Component)
Part for inserting modified D-Dogs for Golden Gate Assembly using BsgI

placIQ RBS
BBa_K193604 Version 1 (Component)
GFP behind a constitutive promoter (placIQ) on pSB4A5

BBa_I731014
BBa_I731014 Version 1 (Component)
The luxR based receiver, F2620 (formerly I13270), controls the production of mCherry

placIQ RBS
BBa_K193601 Version 1 (Component)
Constitutive Promoter (placIQ ) + RBS + melA on Low copy vector(pSB6A1)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K165100
BBa_K165100 Version 1 (Component)
Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS304*

BBa_K165101
BBa_K165101 Version 1 (Component)
Zif268-HIV bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS304*

BBa_K1437006
BBa_K1437006 Version 1 (Component)
mouse AhR detective protein 83-805 with terminator codon optimized for yeast

BBa_K726009
BBa_K726009 Version 1 (Component)
T7 driven lac operated inducer for the rhl quorum-sensing system

BBa_K2066500
BBa_K2066500 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.