BBa_K1053005
BBa_K1053005 Version 1 (Component)
PBAD- TR-HHR*(RBS)-GFPuv-DT

BBa_M11402
BBa_M11402 Version 1 (Component)
5' UTR and RBS of psbA2 gene in Synechocystis sp. PCC 6803

BBa_K1053203
BBa_K1053203 Version 1 (Component)
Pconst.-SRⅡ-HtrⅡ-EnvZ-Pompc-taRNA-DT-Pconst.-HHR-GFP-DT

BBa_K1053202
BBa_K1053202 Version 1 (Component)
Pconst.-SRⅡ-HtrⅡ-EnvZ-Pompc-HHR-GFP-DT-Pconst.-taRNA-DT

BBa_K1053204
BBa_K1053204 Version 1 (Component)
Pconst.-SRⅡ-HtrⅡ-EnvZ-Pompc-taRNA-DT-Pconst.-HHR*-GFP-DT

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.