BBa_K899006
BBa_K899006 Version 1 (Component)
heat inducible FFT with TA system compatible with BBa_K899005

BBa_K137094
BBa_K137094 Version 1 (Component)
GFP device with optimized promoter with (TA) repeat 17bp

BBa_K137093
BBa_K137093 Version 1 (Component)
GFP device with optimized promoter with (TA) repeat 15bp

BBa_K137096
BBa_K137096 Version 1 (Component)
GFP device with optimized promoter with (TA) repeat 21bp

BBa_K137095
BBa_K137095 Version 1 (Component)
GFP device with optimized promoter with (TA) repeat 19bp

luxR-ndh
BBa_K1036000 Version 1 (Component)
lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II

BBa_K726009
BBa_K726009 Version 1 (Component)
T7 driven lac operated inducer for the rhl quorum-sensing system

BBa_K1669000
BBa_K1669000 Version 1 (Component)
CtfA + His-tag

BBa_K137088
BBa_K137088 Version 1 (Component)
optimized (TA) repeat constitutive promoter with 19 bp between -10 and -35 elements

BBa_K137087
BBa_K137087 Version 1 (Component)
optimized (TA) repeat constitutive promoter with 17 bp between -10 and -35 elements

BBa_K137089
BBa_K137089 Version 1 (Component)
optimized (TA) repeat constitutive promoter with 21 bp between -10 and -35 elements

BBa_K137086
BBa_K137086 Version 1 (Component)
optimized (TA) repeat constitutive promoter with 15 bp between -10 and -35 elements

BBa_K137085
BBa_K137085 Version 1 (Component)
optimized (TA) repeat constitutive promoter with 13 bp between -10 and -35 elements

RFPLVA
BBa_K411208 Version 1 (Component)
Red Fluorescent Protein with LVA tag (RFPLVA)

BBa_K1989037
BBa_K1989037 Version 1 (Component)
Triplespytag with mRFP, His-tag , FlAsH-tag and signal peptide PhoA

BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative

BBa_K880004
BBa_K880004 Version 1 (Component)
LVA Tag in RFC25

7x His
BBa_T2001 Version 1 (Component)
7x His tag head domain

c-Myc
BBa_T2006 Version 1 (Component)
c-Myc tail domain (EQKLISEEDL)

BBa_K137064
BBa_K137064 Version 1 (Component)
Promoter with (TA)9 repeat in front of RBS-LacZ-TT

BBa_K137063
BBa_K137063 Version 1 (Component)
Promoter with (TA)10 repeat in front of RBS-LacZ-TT

BBa_K936022
BBa_K936022 Version 1 (Component)
Cutinase and polyhistidine tag

BBa_K831011
BBa_K831011 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K831012
BBa_K831012 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K809321
BBa_K809321 Version 1 (Component)
GAL promoter + YFP_SPtim21 + ADH1 terminator

BBa_K809308
BBa_K809308 Version 1 (Component)
GAL promoter + RFP_SPzim17 + ADH1 terminator

BBa_K1845001
BBa_K1845001 Version 1 (Component)
Miraculin (Yeast codon optimised + His-tag)

BBa_I724005
BBa_I724005 Version 1 (Component)
Elowitz repressilator with added degradation tag

BBa_K1685002
BBa_K1685002 Version 1 (Component)
aeBlue with LVA tag and double terminator

BBa_K2172009
BBa_K2172009 Version 1 (Component)
Tac Promoter-RBS-GST-Thrombin Protease-GFP-Terminator

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_K542011
BBa_K542011 Version 1 (Component)
Catechol 2,3-dioxygenase with C-term Arg-tag (xylE-Arg)

BBa_K1974011
BBa_K1974011 Version 1 (Component)
T7 Promoter+RBS+Hv1a+linker+6X His-Tag

BBa_K1974013
BBa_K1974013 Version 1 (Component)
T7 Promoter+RBS+OAIP+linker+6X His-Tag

BBa_K809710
BBa_K809710 Version 1 (Component)
GAL promoter + kozak + ZIM17 + T7 RNAP + ADH1 terminator

BBa_K1974022
BBa_K1974022 Version 1 (Component)
T7Promoter+RBS+Sf1a+linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1974021
BBa_K1974021 Version 1 (Component)
T7Promoter+RBS+Hv1a+linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1974023
BBa_K1974023 Version 1 (Component)
T7Promoter+RBS+OAIP+linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1036003
BBa_K1036003 Version 1 (Component)
lux pL controlled luxR with lux pR controlled gfp (LVA-tag)

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

BBa_K2123112
BBa_K2123112 Version 1 (Component)
Tac promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)

BBa_K1974033
BBa_K1974033 Version 1 (Component)
T7 Promoter+RBS+Hv1a+GS linker+snowdrop-lectin+linker+6X His-Tag

BBa_K2123115
BBa_K2123115 Version 1 (Component)
Universal promoter (Tac + JK26) for both growth phase with downstream mer operator + K081014

BBa_K2144011
BBa_K2144011 Version 1 (Component)
Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter

BBa_K2066500
BBa_K2066500 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.