BBa_K1039022
BBa_K1039022 Version 1 (Component)
PhiC31 integrase and PhiC31 RDF under lock and key control with lacI Expression Cassette

BBa_K863205
BBa_K863205 Version 1 (Component)
GFP integrated in shuttle vector pECPP11JS (BBa_K863204) for integration in yeast

BBa_M36689
BBa_M36689 Version 1 (Component)
HSE 2x Promoter + Bxb1 Recombinase w/ NLS + SV40 Terminator + attB site + CMV Reverse + attP site

BBa_K863209
BBa_K863209 Version 1 (Component)
BBa_500002 integrated in shuttle vector pECPP11JS (BBa_K863204) for integration in yeast

BBa_K863208
BBa_K863208 Version 1 (Component)
tvel35 integrated in shuttle vector pECPP11JS (BBa_K863204) for integration in yeast

BBa_K863207
BBa_K863207 Version 1 (Component)
tvel5 integrated in shuttle vector pECPP11JS (BBa_K863204) for integration in yeast

BBa_K1039032
BBa_K1039032 Version 1 (Component)
BXB1 att B

MtrB
BBa_K1748001 Version 1 (Component)
Extracelllular metal reducing respiratory system integral outer-membrane β-barrel protein MtrB

BBa_M36427
BBa_M36427 Version 1 (Component)
Recombinase Site 1 (Bxb1 AttB)

BBa_K175050
BBa_K175050 Version 1 (Component)
aTc inducible GFP-LVA generator with integrated I-SceI RS + I-SceI RS + TetR generator

BBa_K112995
BBa_K112995 Version 1 (Component)
BBb1 assembly vector - C/A

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.