BBa_K546546
BBa_K546546 Version 1 (Component)
The Synchroscillator: A Tunable Synchronized Oscillatory System

BBa_K2057006
BBa_K2057006 Version 1 (Component)
Expression of gfp under the regulation of promoter BtI-BtII

BBa_K1514005
BBa_K1514005 Version 1 (Component)
TOR-A TAT signaling domain for bacteria

BBa_K1150041
BBa_K1150041 Version 1 (Component)
uniCAS RNaimer to Bla Target 3

BBa_K1150040
BBa_K1150040 Version 1 (Component)
uniCAS RNAimer to Bla Target 2

BBa_K1150042
BBa_K1150042 Version 1 (Component)
uniCAS RNaimer to Bla Target 4

BBa_J70618
BBa_J70618 Version 1 (Component)
GlnS mutant?

mlr A
BBa_K1432001 Version 1 (Component)
A Synthetic microcystin-degrading Mlr A gene

BBa_K1465208
BBa_K1465208 Version 1 (Component)
Carbonic anhydrase (csoS3) and shell-proteins (csoS14) of the carboxysome of H. neapolitanus

BBa_K539651
BBa_K539651 Version 1 (Component)
promoter (lacI regulated) + a RBS (B0032)+Alss with a RBS (B0030)+ilvC

BBa_K2088012
BBa_K2088012 Version 1 (Component)
MhbRpt is a complex promoter which can be induced by 3-HBA.

BBa_I714001
BBa_I714001 Version 1 (Component)
trwC of R388 ( relaxase or nickase of the R388 conjugative plasmid )

BBa_K1796102
BBa_K1796102 Version 1 (Component)
A chain of CODH/ACS beta subunit

BBa_K1413044
BBa_K1413044 Version 1 (Component)
A fusion of Transposon Plasmid and pSB1C3

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1942001
BBa_K1942001 Version 1 (Component)
This part is a short RNA sequence designed for KRAS gene silencing. It is used for down-regulating K

BBa_K247001
BBa_K247001 Version 1 (Component)
mutant LuxR(Dull)

BBa_K235024
BBa_K235024 Version 1 (Component)
[K235018][K235021] (mCherry generator, ribokey-mediated signal inversion)

BBa_K209022
BBa_K209022 Version 1 (Component)
Aar1 A-B part, PTEN (full length) (Dictyostelium discoideum)

BBa_K784043
BBa_K784043 Version 1 (Component)
Mutant T7 RNAP - T7*

BBa_K1202006
BBa_K1202006 Version 1 (Component)
TAT(Trans-Activator of Transcription) protein

BBa_K656002
BBa_K656002 Version 1 (Component)
Cohesin region of Scaffolding Protein A from Clostridium thermocellum

BBa_K944010
BBa_K944010 Version 1 (Component)
Transcriptional regulator of the cyanide inducible promoter and RBS

BBa_K1216008
BBa_K1216008 Version 1 (Component)
Variant of the wild-type pLuxR promoter with lower sensitivity

BBa_M31539
BBa_M31539 Version 1 (Component)
These are the genes in M13K07 responsible for forming the 'tip' of the phage.

BBa_K1707004
BBa_K1707004 Version 1 (Component)
TetR-ssRA repressor with the promoter of cI

BBa_K1510904
BBa_K1510904 Version 1 (Component)
Truncated lysostaphin coding circuit

BBa_K1725313
BBa_K1725313 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725329
BBa_K1725329 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725327
BBa_K1725327 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725311
BBa_K1725311 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725319
BBa_K1725319 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725318
BBa_K1725318 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K826000
BBa_K826000 Version 1 (Component)
cytochrome which catalyzes the terminal oxidation of alkanes

BBa_K1725322
BBa_K1725322 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725316
BBa_K1725316 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725332
BBa_K1725332 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725328
BBa_K1725328 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725303
BBa_K1725303 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725325
BBa_K1725325 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725315
BBa_K1725315 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725312
BBa_K1725312 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725331
BBa_K1725331 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725314
BBa_K1725314 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725304
BBa_K1725304 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725324
BBa_K1725324 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725323
BBa_K1725323 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1725308
BBa_K1725308 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K883502
BBa_K883502 Version 1 (Component)
TuYV coat protein with part of the readthrough

BBa_K1725326
BBa_K1725326 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032