BBa_I12029
BBa_I12029 Version 1 (Component)
Test of pBAD/araC promoter (I0500)

BBa_I12026
BBa_I12026 Version 1 (Component)
Test of BBa_R0011 (LacI regulated) using YFP

BBa_I13901
BBa_I13901 Version 1 (Component)
Synchronized Brickilator Test Construct (I13201.Q04400.I0461)

BBa_J69122
BBa_J69122 Version 1 (Component)
C12 AHL Receiver test construct with EYFP Reporter

[OriTR]+[R
BBa_K1439002 Version 1 (Component)
This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.