BBa_K2044009
BBa_K2044009 Version 1 (Component)
Based on our project, <4,8> is the direct pathway from Site No.4 to Site No.8 in the map we design.

BBa_K2044014
BBa_K2044014 Version 1 (Component)
Based on our project, <1,4> is the direct pathway from Site No.1 to Site No.4 in the map we design.

BBa_K2044007
BBa_K2044007 Version 1 (Component)
Based on our project, <4,5> is the direct pathway from Site No.4 to Site No.5 in the map we design.

BBa_K2044013
BBa_K2044013 Version 1 (Component)
Based on our project,<7,8> is the direct pathway from Site No.7 to Site No.8 in the map we design.

BBa_K2044008
BBa_K2044008 Version 1 (Component)
Based on our project, <4,6> is the direct pathway from Site No.4 to Site No.6 in the map we design.

BBa_K2044003
BBa_K2044003 Version 1 (Component)
Based on our project, <2,3> is the direct pathway from Site No.2 to Site No.3 in the map we design.

BBa_K2044002
BBa_K2044002 Version 1 (Component)
Based on our project, <2,1> is the direct pathway from Site No.2 to Site No.1 in the map we design.

BBa_K2044005
BBa_K2044005 Version 1 (Component)
Based on our project, <2,6> is the direct pathway from Site No.2 to Site No.6 in the map we design.

BBa_K2044012
BBa_K2044012 Version 1 (Component)
Based on our project, <6,8> is the direct pathway from Site No.6 to Site No.8 in the map we design.

BBa_K2044011
BBa_K2044011 Version 1 (Component)
Based on our project,<6,4> is the direct pathway from Site No.6 to Site No.4 in the map we design.

BBa_K239014
BBa_K239014 Version 1 (Component)
tetR consititutive CFP Device

BBa_J14452
BBa_J14452 Version 1 (Component)
CFP construct (R0011.E0420)

BBa_K541715
BBa_K541715 Version 1 (Component)
Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide

SISO_CN
BBa_K255002 Version 1 (Component)
pTet.lacI-cfp(fusion).terminator.pTet.yfp.terminator

BBa_K594011
BBa_K594011 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL and ECFP reporter.

BBa_K1707028
BBa_K1707028 Version 1 (Component)
CFP-ssRA downstream of a cI regulated promoter

SISO_LacI
BBa_K255003 Version 1 (Component)
pLac.lacI-cfp(fusion).terminator.pTet.yfp.terminator

BBa_K594014
BBa_K594014 Version 1 (Component)
A device that can accepts the 3--OH-C14:1-HSL and then produces 3-O-C6-HSL and GFP reporter.

BBa_K1795020
BBa_K1795020 Version 1 (Component)
CFP-LVA under promoter R0051

BBa_K794001
BBa_K794001 Version 1 (Component)
CSP(derived from E. coli) - lasI

BBa_J13076
BBa_J13076 Version 1 (Component)
Monocistronic CFP/YFP expression cassette

BBa_I13972
BBa_I13972 Version 1 (Component)
Lux CFP Protein Generator (R0062.E0420)

BBa_I13971
BBa_I13971 Version 1 (Component)
c1 CFP Protein Generator (R0051.E0420)

BBa_K1462400
BBa_K1462400 Version 1 (Component)
pGAL1+PRK+GBD-ligand+ADH1+pTDH3+RuBisCo+SH3-ligand+ADH1+pTDH3+CA+PDZ-ligand+ADH1

BBa_I15024
BBa_I15024 Version 1 (Component)
Tet-repressible polycistronic CFP/YFP under B0034

BBa_I13603
BBa_I13603 Version 1 (Component)
Lac operator with CFP reporter (with LVA tag) [R/Lc+]

BBa_K1412088
BBa_K1412088 Version 1 (Component)
A combination of theophylline aptamer and taRNA that can response theophylline to regulate circuit

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.