TerDnaE3(C
BBa_K1362131 Version 1 (Component)
RBS + TerDnaE3 C-intein RFC[105] assembly construct (with His6)

SspDnaB(C)
BBa_K1362141 Version 1 (Component)
RBS + SspDnaB C-intein RFC[105] assembly construct (with His6)

SspDnaX(N)
BBa_K1362110 Version 1 (Component)
RBS + SspDnaX N-intein RFC[105] assembly construct (with His6)

SspGyrB(N)
BBa_K1362120 Version 1 (Component)
RBS + SspGyrB N-intein RFC[105] assembly construct (with His6)

SspGyrB(C)
BBa_K1362121 Version 1 (Component)
RBS + SspGyrB C-intein RFC[105] assembly construct (with His6)

SspDnaE(C)
BBa_K1362411 Version 1 (Component)
SspDnaE C-Intein cloning piece

BBa_K195200
BBa_K195200 Version 1 (Component)
Human alpha-V integrin gene

1NpuE(C*)
BBa_K1362103 Version 1 (Component)
RBS + NpuDnaE C-intein RFC[105] non-slicing control construct (with His6)

BBa_K1362111
BBa_K1362111 Version 1 (Component)
RBS + SspDnaX C-intein RFC[105] assembly construct (with His6)

BBa_K1321120
BBa_K1321120 Version 1 (Component)
SmtA metallothionein fused to linker-dCBD

BBa_J133572
BBa_J133572 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_K608404
BBa_K608404 Version 1 (Component)
IPTG-inducible Promoter with plastic binding domain-tagged GFP

BBa_J133580
BBa_J133580 Version 1 (Component)
Protein Linker SSGSSG Library

Gp41-1(C)
BBa_K1362413 Version 1 (Component)
Gp41-1 C-Intein cloning piece

BBa_K782062
BBa_K782062 Version 1 (Component)
ΔCBD Alginate lyase (mature variant)

Strep-tag
BBa_M0031 Version 1 (Component)
Strep-tag II affinity tag

BBa_M36009
BBa_M36009 Version 1 (Component)
5' Bicistronic UTR (strong), does not include ATG start

BBa_K1491025
BBa_K1491025 Version 1 (Component)
T7 RNA Polymerase with ER Intein

BBa_K1399006
BBa_K1399006 Version 1 (Component)
GFP (mut3b) with LAA-ssrA degradation tag

BBa_K1399008
BBa_K1399008 Version 1 (Component)
GFP (mut3b) with DAS-ssrA degradation tag

BBa_K1362444
BBa_K1362444 Version 1 (Component)
GGG linker with RGK (extein)

BBa_K1435000
BBa_K1435000 Version 1 (Component)
4-HT inducible intein

BBa_K1123012
BBa_K1123012 Version 1 (Component)
His-Tag and Thrombine Cleavage Site

BBa_K1179069
BBa_K1179069 Version 1 (Component)
CAG promoter

BBa_J70628
BBa_J70628 Version 1 (Component)
RFC12 Sce VMA Intein Internal Domain

BBa_K180000
BBa_K180000 Version 1 (Component)
Hybrid promoter (trp & lac regulated -- tac pR)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SV40 NLS
BBa_J63008 Version 1 (Component)
SV40 nuclear localization sequence from SV40; yeast codon optimized

BBa_K1362160
BBa_K1362160 Version 1 (Component)
RBS + Gp41-1 N-intein RFC[105] assembly construct (with His6)

RGK
BBa_K1362434 Version 1 (Component)
RGK (extein)

BBa_K1582029
BBa_K1582029 Version 1 (Component)
Protein Extraction Tag: Linker+sJanus

BBa_K1679022
BBa_K1679022 Version 1 (Component)
KIG, kcoil+intein+gfp

BBa_K1679027
BBa_K1679027 Version 1 (Component)
VMA, a thermosensitive intein

BBa_M36084
BBa_M36084 Version 1 (Component)
Histidine Tag and Stop Codon

BBa_K1679037
BBa_K1679037 Version 1 (Component)
VMA Dead, an inactivated intein

BBa_I13770
BBa_I13770 Version 1 (Component)
Codon CBA

Tat PROM
BBa_K895000 Version 1 (Component)
Eco_Ptat2

BBa_K212001
BBa_K212001 Version 1 (Component)
Tar-Envz (Taz)

BBa_K364308
BBa_K364308 Version 1 (Component)
Human PXR LBD

BBa_K1800003
BBa_K1800003 Version 1 (Component)
CBDA Synthase

BBa_K2027001
BBa_K2027001 Version 1 (Component)
AE Blue-CBD-FlagLumioHisTag Fusion Protein

IBMc318
IBMc318 Version 1 (Component)
pSB1A3-BbsI-G-ER-LBD-S-SapI

BBa_K1491027
BBa_K1491027 Version 1 (Component)
T7 RNA Polymerase with Dead ER Intein

BBa_K1453000
BBa_K1453000 Version 1 (Component)
ssDsbA-mRFP-Lgt-TAL USB-His Tag

BBa_K404112
BBa_K404112 Version 1 (Component)
Cytosine deaminase (CD)

BBa_K1332002
BBa_K1332002 Version 1 (Component)
Histidine tag (8 AA) and RFP (without stop codon)

BBa_K1321348
BBa_K1321348 Version 1 (Component)
sfGFP fused to dCBD with linker in RFC 25

SspDnaB(N*
BBa_K1362142 Version 1 (Component)
RBS + SspDnaB N-intein RFC[105] non-splicing control construct (with His6)

BBa_K1332001
BBa_K1332001 Version 1 (Component)
Histidine tag (8 AA) and RFP

BBa_K1321345
BBa_K1321345 Version 1 (Component)
NiBP fused to dCBD with linker in RFC 25