BBa_K2082237
BBa_K2082237 Version 1 (Component)
RFP and Tetracycline resistence(tetA) under the control of a modified lacZ promoter; Fusion protein

BBa_K1113501
BBa_K1113501 Version 1 (Component)
GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag

BBa_K079052
BBa_K079052 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato

Co-op
BBa_K233002 Version 1 (Component)
Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway

BBa_K233001
BBa_K233001 Version 1 (Component)
Snowdrift 1 , using TorA. A module to secrete B-galactosidase using the TAT-export Pathway

BBa_I716212
BBa_I716212 Version 1 (Component)
Cre (GTG start)

BBa_K079020
BBa_K079020 Version 1 (Component)
GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein

BBa_K422007
BBa_K422007 Version 1 (Component)
CheY (AarI A-part)

SUPPORT DE
BBa_K1433022 Version 1 (Component)
rT-rKan-rRBS-attB-P-attP-RBS-lambda red-RBS-Chl-T-P-RBS-gp47-tag-T

BBa_K1778005
BBa_K1778005 Version 1 (Component)
eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene

Bm3R1
BBa_K1401000 Version 1 (Component)
TetR homolog. This is a MoClo part of the Bm3R1 repressor gene with CD fusion sites ('AATG', 'AGGT')

BBa_K327013
BBa_K327013 Version 1 (Component)
The initiator

BBa_K175035
BBa_K175035 Version 1 (Component)
Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock

BBa_K594015
BBa_K594015 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces lasI and EYFP

BBa_K175034
BBa_K175034 Version 1 (Component)
Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock

BBa_K1113411
BBa_K1113411 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome

BBa_K1189029
BBa_K1189029 Version 1 (Component)
TALE-A with a his tag linked to a K coil under the control of a LacI promoter

BBa_K1778002
BBa_K1778002 Version 1 (Component)
TRE-CYC1TATA is a recombinant promoter, which is constructed in order to make the Tet-on system func

BBa_K1361003
BBa_K1361003 Version 1 (Component)
Curli Fiber generator under the control of T7 promoter with a relatively strong expression of CsgA,C

BBa_M31513
BBa_M31513 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

BBa_M31516
BBa_M31516 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

BBa_K1361001
BBa_K1361001 Version 1 (Component)
Curli Fiber generator under the control of T7 promoter with a relatively weak expression of CsgA,C

BBa_I10074
BBa_I10074 Version 1 (Component)
CheY Protein Generator

BBa_K2044000
BBa_K2044000 Version 1 (Component)
Based on our project, <2-6-8> is the optimal pathway scheme from Site No. 2 to Site No. 8

BBa_K557005
BBa_K557005 Version 1 (Component)
Reversed Toggle switch+Aptamer-CheZ

BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative

BBa_K2082252
BBa_K2082252 Version 1 (Component)
RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI

BBa_K1947028
BBa_K1947028 Version 1 (Component)
Improve the protein purification.

BBa_K1947027
BBa_K1947027 Version 1 (Component)
Improve the protein purification.

BBa_K1947026
BBa_K1947026 Version 1 (Component)
Improve the protein purification.

BBa_K886002
BBa_K886002 Version 1 (Component)
Recombination Device composed by Cre-lox71

BBa_K594011
BBa_K594011 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL and ECFP reporter.

BBa_K594014
BBa_K594014 Version 1 (Component)
A device that can accepts the 3--OH-C14:1-HSL and then produces 3-O-C6-HSL and GFP reporter.

Q04510+RFP
BBa_K131018 Version 1 (Component)
Intermediate for the Response circuit

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_I20292
BBa_I20292 Version 1 (Component)
There is no limit to what a man can do or where he can go if...

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1351017
BBa_K1351017 Version 1 (Component)
SdpI with RBS: Immunity against the cannibalsim toxin sdpC of <i>B. subtilis</i>

BBa_K1114003
BBa_K1114003 Version 1 (Component)
The MoClo format of BBa_J23103 with AB fusion sites.

BBa_M36914
BBa_M36914 Version 1 (Component)
Codes for the benzylalcohol acetyltransferase enzyme

BBa_K2012021
BBa_K2012021 Version 1 (Component)
PcpcG2-172-B0034-CheZ-GS3-GFP

BBa_K1593003
BBa_K1593003 Version 1 (Component)
Chemotaxis related protein cheZ from Pseudomonas aeruginosa

BBa_K1947017
BBa_K1947017 Version 1 (Component)
We verify the expression effect of Mms13.

BBa_K1051262
BBa_K1051262 Version 1 (Component)
The measurement pathway of degradation tag K1051208.

BBa_K1051260
BBa_K1051260 Version 1 (Component)
The measurement pathway of degradation tag K1051206.

BBa_K1051261
BBa_K1051261 Version 1 (Component)
The measurement pathway of degradation tag K1051207.

BBa_K1323020
BBa_K1323020 Version 1 (Component)
oriV from the Staphylococcus aureus pSK41 plasmid clone 3