BBa_K654041
BBa_K654041 Version 1 (Component)
Synechocystis sigA promoter controlled Firefly Luciferase

BBa_K654093
BBa_K654093 Version 1 (Component)
Synechocystis psbA2 5'UTR Native RBS + Renilla Luciferase

BBa_K654094
BBa_K654094 Version 1 (Component)
Synechocystis psbA2 5'UTR Consensus RBS + Renilla Luciferase

BBa_K654043
BBa_K654043 Version 1 (Component)
psbA2 5'UTR Native RBS + Firefly Luciferase + Terminator

BBa_K654044
BBa_K654044 Version 1 (Component)
Synechocystis psbA2 5'UTR Consensus RBS + Firefly Luciferase

BBa_K654045
BBa_K654045 Version 1 (Component)
Promoter Tester Anderson E.coli 1.0 Reference Dual Luciferase Assay

BBa_K654046
BBa_K654046 Version 1 (Component)
Promoter/RBS Tester Anderson 1.0 Reference Dual Luciferase Assay

BBa_K654047
BBa_K654047 Version 1 (Component)
Promoter Control Anderson E.coli 1.0 Reference Dual Luciferase Assay

BBa_K654048
BBa_K654048 Version 1 (Component)
Promoter Tester Native 6803 RBS psbA2 Reference Dual Luciferase Assay

BBa_K654049
BBa_K654049 Version 1 (Component)
Promoter/RBS Tester psbA2 Reference Dual Luciferase Assay

BBa_K654050
BBa_K654050 Version 1 (Component)
Promoter Control psbA2 Reference Dual Luciferase Assay

BBa_K654054
BBa_K654054 Version 1 (Component)
Promoter Tester 6803 Consensus RBS psbA2 Reference Dual Luciferase Assay

BBa_K654051
BBa_K654051 Version 1 (Component)
Promoter Tester 6803 Native RBS sigA Reference Dual Luciferase Assay

BBa_K654052
BBa_K654052 Version 1 (Component)
Promoter/RBS Tester sigA Reference Dual Luciferase Assay

BBa_K654053
BBa_K654053 Version 1 (Component)
Promoter Control sigA Reference Dual Luciferase Assay

BBa_K654055
BBa_K654055 Version 1 (Component)
Promoter Tester 6803 Consensus RBS sigA Reference Dual Luciferase Assay

BBa_K654074
BBa_K654074 Version 1 (Component)
psbA2 controlled Fatty ACP Reductase

BBa_K896000
BBa_K896000 Version 1 (Component)
SQR(sulfide quinone reductase),from Synechococcus sp. PCC 7002 plasmid pAQ7

BBa_K823051
BBa_K823051 Version 1 (Component)
mKate2, a red monomeric fluorescent protein + RBS + Term

BBa_K936017
BBa_K936017 Version 1 (Component)
K206000+B0034+Reductase (anaerobic)+B0034+Dehydrogenase

BBa_K936018
BBa_K936018 Version 1 (Component)
B0034+Reductase+B0015

BBa_K896003
BBa_K896003 Version 1 (Component)
Sulfide quinone reductase(SQR) on neutral site 2 vector

BBa_K823050
BBa_K823050 Version 1 (Component)
luc luciferase, codon optimized for Bacillus subtilis+ RBS+Term

BBa_K897318
BBa_K897318 Version 1 (Component)
Paired termini structure

TBY
BBa_K897720 Version 1 (Component)
Antisense FtsZ protected by paired termini structure under T7 promoter

BBa_K759005
BBa_K759005 Version 1 (Component)
phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)

BBa_K782019
BBa_K782019 Version 1 (Component)
10x[TALA+TALB] operator_CMV promoter_fLuciferase

BBa_K782020
BBa_K782020 Version 1 (Component)
10x[TALA] operator_CMV promoter_fLuciferase

BBa_K782021
BBa_K782021 Version 1 (Component)
10x[TALB] operator_CMV promoter_fLuciferase

BBa_K782022
BBa_K782022 Version 1 (Component)
12x[TALD] operator_CMV promoter_fLuciferase

BBa_K782023
BBa_K782023 Version 1 (Component)
12x[NicTAL] operator_CMV promoter_fLuciferase

BBa_K759007
BBa_K759007 Version 1 (Component)
RBS(B0034)and phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K782032
BBa_K782032 Version 1 (Component)
10x[TALA+TALB] operator_minimal promoter_fLuciferase

BBa_K782033
BBa_K782033 Version 1 (Component)
10x[TALA] operator_minimal promoter_fLuciferase

Tm-Split-L
BBa_K157020 Version 1 (Component)
Tm-Split-Luciferase1/2

Tm-Splitlu
BBa_K157021 Version 1 (Component)
Tm-Splitluciferase2/2

SP-AntiNIP
BBa_K157030 Version 1 (Component)
SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2

SP-AntiNIP
BBa_K157031 Version 1 (Component)
SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2

SP-Lipocal
BBa_K157038 Version 1 (Component)
SP-Lipocalin-GGGSlinker-Tm-Splitluciferase2/2

SP-Lipocal
BBa_K157039 Version 1 (Component)
SP-Lipocalin-GGGSlinker-Tm-Splitluciferase1/2

Bioplastic
BBa_K156013 Version 1 (Component)
phaB1 (acetyacetyl-CoA reductase)

BBa_K116201
BBa_K116201 Version 1 (Component)
ureD promoter from <I>P mirabilis<I>

BBa_J24817
BBa_J24817 Version 1 (Component)
dLaRAP firefly luciferase reporter device for promoters

BBa_J24818
BBa_J24818 Version 1 (Component)
dLaRAP firefly luciferase reporter device for promoters

BBa_J24819
BBa_J24819 Version 1 (Component)
dLaRAP firefly luciferase reporter device for promoters with J23101

BBa_J24820
BBa_J24820 Version 1 (Component)
Renilla luciferase reporter device for DLARAP

BBa_M11006
BBa_M11006 Version 1 (Component)
ArsC arsenate reductase protein

BBa_M11010
BBa_M11010 Version 1 (Component)
Thioredoxin Peroxidase II, Cricetulus griseus, mRNA Coding