BBa_M1986
BBa_M1986 Version 1 (Component)
rpsT gene from Methylobacterium Chloromethanicum

BBa_K1725081
BBa_K1725081 Version 1 (Component)
T1 terminator from E. coli rrnB

BBa_K1172904
BBa_K1172904 Version 1 (Component)
Rnase Ba (Barnase) from Bacillus amyloliquefaciens

BBa_K1315007
BBa_K1315007 Version 1 (Component)
The BCAM0228 Response Regulator from <i>Burkholderia</i>

Rec-Open
BBa_I726081 Version 1 (Component)
Rec-LRY.RC

BBa_K617003
BBa_K617003 Version 1 (Component)
R6K origin of replication

BBa_I11010
BBa_I11010 Version 1 (Component)
RBS

BBa_J29028
BBa_J29028 Version 1 (Component)
rod shape-determining protein

BBa_K1172502
BBa_K1172502 Version 1 (Component)
Outer membrane porin OprF from Pseudomonas fluorescens with PT7 and RBS

BBa_J64001
BBa_J64001 Version 1 (Component)
psicA from <I>Salmonella</I>

BBa_K1462330
BBa_K1462330 Version 1 (Component)
RuBisCo (from Thiobacillus denitrificans)

BBa_K1689006
BBa_K1689006 Version 1 (Component)
Coding sequence of FKBP-Cluc394

ninaB
BBa_K343001 Version 1 (Component)
B-carotene monooxygenase, Produces retinal from B-carotene. ninaB gene from Drosophila.

BBa_K116601
BBa_K116601 Version 1 (Component)
HtlB (ftsH) coding region from E. coli

BBa_K116000
BBa_K116000 Version 1 (Component)
A homolog of OCT3 (organic cation transporter 3) from E coli. K12

BBa_K112707
BBa_K112707 Version 1 (Component)
Pspv from <I>Salmonella</I>

BBa_I728456
BBa_I728456 Version 1 (Component)
MerRT: Mercury-Inducible Promoter+RBS (MerR + part of MerT)

BBa_K2066503
BBa_K2066503 Version 1 (Component)
8BP Spacer from UNSX

BBa_K1689005
BBa_K1689005 Version 1 (Component)
Coding sequence of FKBP-Cluc398

BBa_K1689002
BBa_K1689002 Version 1 (Component)
Coding sequence of Cluc-STV

BBa_K1758360
BBa_K1758360 Version 1 (Component)
gabR gene and gabT promoter from Bacillus subtilis

BBa_K415003
BBa_K415003 Version 1 (Component)
P_const(low/med) : RBS : LuxR : Term : pLux/CI-OR

BBa_E2020
BBa_E2020 Version 1 (Component)
enhanced version of ECFP, yeast-optimized

SPtom70
BBa_K809205 Version 1 (Component)
signal peptide of Tom70

BBa_I11030
BBa_I11030 Version 1 (Component)
integrase from E. coli phage P22

BBa_S03634
BBa_S03634 Version 1 (Component)
RBS-TetF : HixC-TT-RE

BBa_K188222
BBa_K188222 Version 1 (Component)
RBS+cI repressor from phage 434 (+LVA)

BBa_K188122
BBa_K188122 Version 1 (Component)
RBS+lacI repressor from E. coli (+LVA)

BBa_K1392936
BBa_K1392936 Version 1 (Component)
RBS + Tetracycline Repressor from Transposon Tn10 (LVA)

BBa_K518012
BBa_K518012 Version 1 (Component)
RBS + RFP + d.Ter

UPcox1
BBa_K809003 Version 1 (Component)
Up elements of cox1 gene

BBa_K861500
BBa_K861500 Version 1 (Component)
FadL, Long-chain fatty acids transporter from <i>E.coli str. K12</i>

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1911003
BBa_K1911003 Version 1 (Component)
ClpX from E. Coli

BBa_K1911002
BBa_K1911002 Version 1 (Component)
ClpP from E. coli

BBa_K797001
BBa_K797001 Version 1 (Component)
phage shock protein A: one of the components of out Tat cassette

BBa_M36627
BBa_M36627 Version 1 (Component)
GFP (modified from BBa_K648013)

BBa_J70102
BBa_J70102 Version 1 (Component)
Left 6 his test part for library construction part.

BBa_J70104
BBa_J70104 Version 1 (Component)
Right 6 his test part for library construction part.

BBa_K1332003
BBa_K1332003 Version 1 (Component)
The 5?? side of the intron(+exon fragment) from td gene of T4 phage without stop codon

BBa_J22102
BBa_J22102 Version 1 (Component)
rec A (SOS) Promoter