BBa_S01936
BBa_S01936 Version 1 (Component)
Intermediate part from assembly 240

BBa_S02107
BBa_S02107 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01996
BBa_S01996 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01376
BBa_S01376 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01379
BBa_S01379 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01663
BBa_S01663 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01660
BBa_S01660 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01898
BBa_S01898 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01381
BBa_S01381 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01830
BBa_S01830 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01845
BBa_S01845 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01380
BBa_S01380 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01377
BBa_S01377 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01842
BBa_S01842 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01383
BBa_S01383 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01843
BBa_S01843 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01378
BBa_S01378 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01826
BBa_S01826 Version 1 (Component)
Intermediate part from assembly 240

BBa_S01382
BBa_S01382 Version 1 (Component)
Intermediate part from assembly 239

BBa_S01821
BBa_S01821 Version 1 (Component)
Intermediate part from assembly 240

BBa_S02001
BBa_S02001 Version 1 (Component)
Intermediate part from assembly 240

KanR-BA-Bf
BBa_K349012 Version 1 (Component)
KanR-BA-BfuAI (for construction of BA BioBytes 2.0)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.