BBa_K1053120
BBa_K1053120 Version 1 (Component)
RNA scaffold containing HTLV-1 Rex peptide and λN peptide aptamer

BBa_M34014
BBa_M34014 Version 1 (Component)
Nucleotide sequence of a cDNA clone of saxiphilin from the liver of Rana Catesbeiana

BBa_K2082006
BBa_K2082006 Version 1 (Component)
Nanobody library: Fusion proteins of Nanobody and omega subunit of RNA polymerase (rpoZ)

BBa_K1551000
BBa_K1551000 Version 1 (Component)
To generate delta-4 fatty acid Desaturase so as to synthesize the DHA.

BBa_K2082005
BBa_K2082005 Version 1 (Component)
Monobody library: Fusion proteins of Monobody and omega subunit of RNA polymerase (rpoZ)

BBa_K539673
BBa_K539673 Version 1 (Component)
a RBS (B0032)+ilvC+a RBS (B0032)+ilvD with RNA thermometer+tetR+terminator

BBa_K539674
BBa_K539674 Version 1 (Component)
a RBS (B0030)+ilvC+a RBS (B0030)+ilvD with RNA thermometer+tetR+terminator

BBa_K187254
BBa_K187254 Version 1 (Component)
folD, ORF, forward primer

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_K1753000
BBa_K1753000 Version 1 (Component)
the omega subunit of E.coli RNA polymerase with T7 promoter, RBS and terminator(BBa_B0014)

BBa_K1706007
BBa_K1706007 Version 1 (Component)
T7 RNA Polymerase gene

BBa_K831014
BBa_K831014 Version 1 (Component)
Inducible istR (inhibitor of SOS-induced toxicity by RNA) under the control of lac promoter

BBa_K539691
BBa_K539691 Version 1 (Component)
promoter(lacI regulated)+Alss+ilvC+ilvD(each preceded by own RBS)and RNA thermometer+terminator

BBa_J31016
BBa_J31016 Version 1 (Component)
part produces the RNA construct crRNA-RBS-GFPLVA-tt that can only be translated in the presence of t

BBa_K1100076
BBa_K1100076 Version 1 (Component)
RNA-IN S49

BBa_K1864001
BBa_K1864001 Version 1 (Component)
T7 RNA Polymerase

BBa_K1132000
BBa_K1132000 Version 1 (Component)
T7 RNA Polymerase

RNAcj
BBa_K1826005 Version 1 (Component)
RNA thermometer + cjBlue

BBa_J22000
BBa_J22000 Version 1 (Component)
DnaA binding sequence - Px

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

BBa_K1777012
BBa_K1777012 Version 1 (Component)
sequence-specific RNA binding protein

BBa_K1777014
BBa_K1777014 Version 1 (Component)
sequence-specific RNA binding protein

BBa_K1777013
BBa_K1777013 Version 1 (Component)
sequence-specific RNA binding protein

BBa_K1777004
BBa_K1777004 Version 1 (Component)
sequence-specific RNA binding protein

BBa_K2050419
BBa_K2050419 Version 1 (Component)
RNA Polimerase III Promoter (H1 promoter)

BBa_K1824559
BBa_K1824559 Version 1 (Component)
RNA Thermometer FourU (Specially designed for J23119)

BBa_K1824558
BBa_K1824558 Version 1 (Component)
RNA Thermometer A1 (Specially designed for T7)

BBa_J92006
BBa_J92006 Version 1 (Component)
M. Ruminantium RNA polymerase Subunit A"

BBa_K2050422
BBa_K2050422 Version 1 (Component)
Mouse H1 promoter (RNA Polymerase III promoter)

BBa_K1363604
BBa_K1363604 Version 1 (Component)
key of no of RNA logic gates

BBa_K779117
BBa_K779117 Version 1 (Component)
RNA Reporter top strand with quencher (RQ) MammoBlock

BBa_K2020051
BBa_K2020051 Version 1 (Component)
wild type tyrosyl synthetase for use in E.coli with amber anticodon and Y32G

BBa_K581003
BBa_K581003 Version 1 (Component)
SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)

BBa_K779116
BBa_K779116 Version 1 (Component)
Short RNA reporter bottom strand (with ROX fluorophore) MammoBlock

BBa_K779120
BBa_K779120 Version 1 (Component)
RNA Reporter top strand with quencher (RQ) and tag fluorophore (Alexa 488) MammoBlock

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K831011
BBa_K831011 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K831012
BBa_K831012 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K1942001
BBa_K1942001 Version 1 (Component)
This part is a short RNA sequence designed for KRAS gene silencing. It is used for down-regulating K

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.