BBa_K137021
BBa_K137021 Version 1 (Component)
GFP with (AC)20 repeat after start codon

BBa_K775012
BBa_K775012 Version 1 (Component)
diacetyl chimeric GPCR with Fus1-EGFP reporter (2)

BBa_K988001
BBa_K988001 Version 1 (Component)
K314100_PelB signal sequence_Oxytocin-neurophysin1_6x_His-tag_stop codon

BBa_K1695042
BBa_K1695042 Version 1 (Component)
Riboswitch Bacteriophage 21 Codon Optimized Lysis Cassette S R Rz

BBa_K137033
BBa_K137033 Version 1 (Component)
Device with GFP with (AC)21 repeat after start codon

BBa_K1695049
BBa_K1695049 Version 1 (Component)
pL8-UV5 + Riboswitch Bacteriophage 21 Codon Optimized Lysis Cassette S R Rz

BBa_K779121
BBa_K779121 Version 1 (Component)
Short DNA reporter top strand (with RQ quencher) MammoBlock

BBa_K779120
BBa_K779120 Version 1 (Component)
RNA Reporter top strand with quencher (RQ) and tag fluorophore (Alexa 488) MammoBlock

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.