ZF test
BBa_J05000 Version 1 (Component)
Zinc Finger Protein Tester

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1159110
BBa_K1159110 Version 1 (Component)
Thermonuclease with N-terminal SV40 NLS and flexible linker with TEV site in RFC[25]

BBa_S03515
BBa_S03515 Version 1 (Component)
Hin : TT

BBa_M50043
BBa_M50043 Version 1 (Component)
HIV-1 U3 Region

BBa_M50044
BBa_M50044 Version 1 (Component)
HIV-1 U5 Region

BBa_K1022103
BBa_K1022103 Version 1 (Component)
pT7: RBS: His6- SUMO: Magainin II

BBa_K1897006
BBa_K1897006 Version 1 (Component)
Has operon promoter

BBa_K1418002
BBa_K1418002 Version 1 (Component)
Chlorophyllase (TaCHL) generator with C-terminal 10x His tag

BBa_K329016
BBa_K329016 Version 1 (Component)
RBS + Xis-Int Lambda

BBa_K329018
BBa_K329018 Version 1 (Component)
RBS + Xis-Int Tn916

His_FluA
BBa_S04282 Version 1 (Component)
K157011(His affinity tag; Freiburg standard):K157004(Fluorescin-A-binding )

BBa_K2012009
BBa_K2012009 Version 1 (Component)
RBS(J61100) and CheZ with fused with a GS linker and GFP in C teminal

BBa_K883503
BBa_K883503 Version 1 (Component)
TuYV coat protein with part of the readthrough with His-tag

BBa_J61054
BBa_J61054 Version 1 (Component)
[HIP-1] Promoter

BBa_J61055
BBa_J61055 Version 1 (Component)
[HIP-1fnr] Promoter

BBa_K1055000
BBa_K1055000 Version 1 (Component)
mKate is a fluorophor derived from dsRed

BBa_K1831000
BBa_K1831000 Version 1 (Component)
NpuDnaE intein for circularization of Type lll AFP+ inker

BBa_K792011
BBa_K792011 Version 1 (Component)
Yeast exportable His-rich peptide w/enhanced import (2)

BBa_K362004
BBa_K362004 Version 1 (Component)
sodA promoter is controlled by some transcription factors including SoxS.

BBa_K1988005
BBa_K1988005 Version 1 (Component)
his-tagged Pit B homologue w/ Anderson Promoter J23106

BBa_K1988011
BBa_K1988011 Version 1 (Component)
his-tagged PPGK ATPi homologue w/ Anderson Promoter J23106

BBa_K1988007
BBa_K1988007 Version 1 (Component)
his-tagged Pit C homologue w/ Anderson Promoter J23106

BBa_K1988003
BBa_K1988003 Version 1 (Component)
his-tagged Pit A homologue w/ Anderson Promoter J23106

BBa_S03383
BBa_S03383 Version 1 (Component)
Hix Left Site

BBa_S03384
BBa_S03384 Version 1 (Component)
Hix Right Site

BBa_K1493602
BBa_K1493602 Version 1 (Component)
pLacI + Kis Antitoxin (Kis-Kid Toxin-Antitoxin System)

BBa_K1231000
BBa_K1231000 Version 1 (Component)
The asr promoter is a pH-responsive promoter.

BBa_I11041
BBa_I11041 Version 1 (Component)
HK022 Xis +AAV

BBa_K1088059
BBa_K1088059 Version 1 (Component)
GFP reporter with flexible linker at N-terminus for creation of GFP fusions

BBa_T1106
BBa_T1106 Version 1 (Component)
Kill this part

BBa_K165064
BBa_K165064 Version 1 (Component)
Zif268-HIV activator

BBa_T1105
BBa_T1105 Version 1 (Component)
Kill this part

BBa_T1100
BBa_T1100 Version 1 (Component)
Kill this part

BBa_J64707
BBa_J64707 Version 1 (Component)
hisH from B. subtilis

BBa_J70563
BBa_J70563 Version 1 (Component)
J04430 with C-terminal 6 his tag and no terminator

BBa_K1189000
BBa_K1189000 Version 1 (Component)
TALE-A with a his tag under a lacI promoter

BBa_K1189017
BBa_K1189017 Version 1 (Component)
K coil and 6X His Tag under inducible LacI promoter

BBa_K533003
BBa_K533003 Version 1 (Component)
OmpA-HIV aspartyl protease

TALEA-dife
BBa_K1189021 Version 1 (Component)
TALE-A ferritin di-subunit direct fusion w/ hisTag under lacI promoter, and linker

BBa_K157010
BBa_K157010 Version 1 (Component)
glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac

BBa_K543000
BBa_K543000 Version 1 (Component)
HNS protein (wild type)

BBa_K1932007
BBa_K1932007 Version 1 (Component)
This device is constructed for the expression of TAT-apoptin fused with tmp1.

BBa_J70657
BBa_J70657 Version 1 (Component)
N-terminal 6 his fusion test for GFP J70610w604 inserts

BBa_K615001
BBa_K615001 Version 1 (Component)
Zinc finger-omega subunit fusion protein

BBa_K323054
BBa_K323054 Version 1 (Component)
HIVC link

BBa_T3333
BBa_T3333 Version 1 (Component)
Kill this part

BBa_T1007
BBa_T1007 Version 1 (Component)
Kill this part

037RPMGFPT
BBa_K733007 Version 1 (Component)
<i>Pveg</i> + spoVG RBS + <i>lytC</i> + linker + RPMrel + consensus RBS + GFP + double terminator