BBa_K2152003BBa_K2152003 Version 1 (Component)Bacteriophage Phi X 174 lysis gene E(wild type)
BBa_K1363200BBa_K1363200 Version 1 (Component)Anti-LPS factor(LALF) regulated by lacI
BBa_K1441012BBa_K1441012 Version 1 (Component)DNA ligase from Escherichia coli with His-tag In pGAPz alpha A
PrtDEFBBa_K258007 Version 1 (Component)Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi
BBa_K831011BBa_K831011 Version 1 (Component)istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12
BBa_K831012BBa_K831012 Version 1 (Component)istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12
BBa_K2092004BBa_K2092004 Version 1 (Component)alcR (incl RBS), ethanol-activated transcription factor from A. nidulans
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.