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Public
BBa_S05123_sequence
BBa_S05123_sequence Version 1 (Sequence)

Public
BBa_S05121_sequence
BBa_S05121_sequence Version 1 (Sequence)

Public
BBa_S05124_sequence
BBa_S05124_sequence Version 1 (Sequence)

Public
BBa_S05122_sequence
BBa_S05122_sequence Version 1 (Sequence)

Public
BBa_S05120_sequence
BBa_S05120_sequence Version 1 (Sequence)

Public
BBa_S05212_sequence
BBa_S05212_sequence Version 1 (Sequence)

Public
BBa_S05012_sequence
BBa_S05012_sequence Version 1 (Sequence)

Public
BBa_S05128_sequence
BBa_S05128_sequence Version 1 (Sequence)

Public
BBa_S05112_sequence
BBa_S05112_sequence Version 1 (Sequence)

Public
BBa_S05129_sequence
BBa_S05129_sequence Version 1 (Sequence)

Public
BBa_S05126_sequence
BBa_S05126_sequence Version 1 (Sequence)

Public
BBa_S05127_sequence
BBa_S05127_sequence Version 1 (Sequence)

Public
SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
Showing 901 - 913 of 913 result(s)
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