BBa_Q200714
BBa_Q200714 Version 1 (Component)
Dimeric zinc finger based inverter

BBa_Q200614
BBa_Q200614 Version 1 (Component)
Dimeric zinc finger based inverter

BBa_Q20070
BBa_Q20070 Version 1 (Component)
Dimeric zinc finger based inverter

BBa_K323061
BBa_K323061 Version 1 (Component)
YFP link CFP

Subtilin P
BBa_K104001 Version 1 (Component)
Sensor for small peptide Subtilin

BBa_K1777003
BBa_K1777003 Version 1 (Component)
miR-21 linear sponge

P-RBS-CWB-
BBa_K316034 Version 1 (Component)
Pveg-spoVG-LytC-Helical Linker-TEV Cleavage Site-AIP-His Tag

P-RBS-CWB-
BBa_K316037 Version 1 (Component)
Pveg-spoVG-LytC-Helical Linker-Elastase Cleavage Site-AIP-His Tag

P-RBS-CWB-
BBa_K316035 Version 1 (Component)
Pveg-spoVG-LytC-Glycine Linker-Elastase Cleavage Site-AIP-His Tag

P-RBS-CWB-
BBa_K316033 Version 1 (Component)
Pveg-spoVG-LytC-Flexible Linker-TEV Cleavage Site-AIP-His Tag

P-RBS-CWB-
BBa_K316036 Version 1 (Component)
Pveg-spoVG-LytC-Flexible Linker-Elastase Cleavage Site-AIP-His Tag

P-RBS-CWB-
BBa_K316032 Version 1 (Component)
Pveg-spoVG-LytC-Glycine Linker-TEV Cleavage Site-AIP-His Tag

BBa_K404158
BBa_K404158 Version 1 (Component)
pCMV_Z-EGFR-1907_SEG-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)

BBa_K404156
BBa_K404156 Version 1 (Component)
pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)

BBa_K404162
BBa_K404162 Version 1 (Component)
pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-BAP)

BBa_K404155
BBa_K404155 Version 1 (Component)
pCMV_Z-EGFR-1907_Short-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)

BBa_K404157
BBa_K404157 Version 1 (Component)
pCMV_Z-EGFR-1907_Long-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)

BBa_K300083
BBa_K300083 Version 1 (Component)
Phasins (separated by a flexible protein domain linker) with intein downstream

BBa_K1321347
BBa_K1321347 Version 1 (Component)
dCBD with N-terminal linker in RFC 25

BBa_K633014
BBa_K633014 Version 1 (Component)
AraC+Pbad promoter+RBS+signal peptide phoA+Cellulase+Linker+estA membrane protein

BBa_J70025
BBa_J70025 Version 1 (Component)
Promoter for tetM gene, from pBOT1 plasmid, pAMbeta1

BBa_K887021
BBa_K887021 Version 1 (Component)
Gli1(zinc finger)+kivd

BBa_K2144009
BBa_K2144009 Version 1 (Component)
Sequence for His6-tag and LPXTGG-tag

creD
BBa_J64905 Version 1 (Component)
creD inner membrane protein

BBa_M1716
BBa_M1716 Version 1 (Component)
Hepcidin Optimized for E. coli

BBa_K1840004
BBa_K1840004 Version 1 (Component)
mCherry for Pseudomonas

BBa_K1615058
BBa_K1615058 Version 1 (Component)
Heroin esterase fused to CBDcenA+Linker in RFC25 driven by LacI promoter

BBa_K195403
BBa_K195403 Version 1 (Component)
This part is composed of pTetR,RBS,Lpp-OmpA-linker and F10scFv

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K299802
BBa_K299802 Version 1 (Component)
Aactag - like a biobrick scar but first T changed to A

BBa_K415117
BBa_K415117 Version 1 (Component)
RBS Weak : pVIII-Fos

BBa_K1180003
BBa_K1180003 Version 1 (Component)
Fusion protein of lamp 2b and pre-s1 for liver targeting of the exosome

BBa_K1638035
BBa_K1638035 Version 1 (Component)
hTrx-based scaffold fused to T18 through intein and a flexible linker

TtrpC
BBa_K678036 Version 1 (Component)
trpC terminator for Aspergillus nidulans

BBa_K133074
BBa_K133074 Version 1 (Component)
CMV-SS-gyrHP-2xlinker

BBa_K133077
BBa_K133077 Version 1 (Component)
CMV-SS-gyrEC-2xlinker

BBa_K1613004
BBa_K1613004 Version 1 (Component)
To detect toxicant harmful to human liver

BBa_K404163
BBa_K404163 Version 1 (Component)
pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-His-Tag)

ZF test
BBa_J05000 Version 1 (Component)
Zinc Finger Protein Tester

BBa_K415141
BBa_K415141 Version 1 (Component)
RBS + M13 p8-Fos fusion

BBa_K1159110
BBa_K1159110 Version 1 (Component)
Thermonuclease with N-terminal SV40 NLS and flexible linker with TEV site in RFC[25]

BBa_J95023
BBa_J95023 Version 1 (Component)
promoter designed for Rhodobacter sphaeroides

T7 express
BBa_K1639012 Version 1 (Component)
T7 expression system for pSB1C3

BBa_K1445000
BBa_K1445000 Version 1 (Component)
M13 Ori: Packaging signal for M13 phage

BBa_K2180006
BBa_K2180006 Version 1 (Component)
PLacO - Promoter LacO for B. subtilis

BBa_K2012009
BBa_K2012009 Version 1 (Component)
RBS(J61100) and CheZ with fused with a GS linker and GFP in C teminal

BBa_M1025
BBa_M1025 Version 1 (Component)
RBS and HIV Zn finger and PhaA pathway

rhII
BBa_C0170 Version 1 (Component)
autoinducer synthetase for N-butyryl-HSL (BHL) and HHL (no LVA)

pMam
BBa_K678023 Version 1 (Component)
Plasmid-Mam for Plug

BBa_I741018
BBa_I741018 Version 1 (Component)
Right facing promoter (for xylF) controlled by xylR and CRP-cAMP