BBa_K1438024
BBa_K1438024 Version 1 (Component)
ATPCS Expression Device

BBa_K317004
BBa_K317004 Version 1 (Component)
Phototaxis Device (Atractant)

BBa_K594015
BBa_K594015 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces lasI and EYFP

BBa_K749010
BBa_K749010 Version 1 (Component)
Formate dehydrogenase device

BBa_I729009
BBa_I729009 Version 1 (Component)
Time device

BBa_K749013
BBa_K749013 Version 1 (Component)
Formate dehydrogenase device

BBa_J14430
BBa_J14430 Version 1 (Component)
GFP coding device

BBa_K1956026
BBa_K1956026 Version 1 (Component)
Noise in Device measurement

BBa_K1956025
BBa_K1956025 Version 1 (Component)
Noise in Device measurement

BBa_K1956024
BBa_K1956024 Version 1 (Component)
Noise in Device measurement

BBa_K1956027
BBa_K1956027 Version 1 (Component)
Noise in Device measurement

BBa_M31114
BBa_M31114 Version 1 (Component)
M13, gene II from HpaI to the end [1-831bp]

BBa_K987001
BBa_K987001 Version 1 (Component)
This is a composite part which has the function to invert the temperature activation by the part: BB

BBa_K091135
BBa_K091135 Version 1 (Component)
Las Sender Testing Device

BBa_K239013
BBa_K239013 Version 1 (Component)
tetR consititutive YFP Device

BBa_I3510
BBa_I3510 Version 1 (Component)
Quorum receiver device (R0063.I0462.R0062)

BBa_K886002
BBa_K886002 Version 1 (Component)
Recombination Device composed by Cre-lox71

BBa_K594011
BBa_K594011 Version 1 (Component)
A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL and ECFP reporter.

BBa_K594014
BBa_K594014 Version 1 (Component)
A device that can accepts the 3--OH-C14:1-HSL and then produces 3-O-C6-HSL and GFP reporter.

BBa_K317033
BBa_K317033 Version 1 (Component)
3OC6HSL Regurated T4 Lysis device

BBa_K317032
BBa_K317032 Version 1 (Component)
IPTG regulated T4 Lysis device

BBa_K1954007
BBa_K1954007 Version 1 (Component)
Green Light Inducible bacteriocin Device (GLID)

BBa_K779603
BBa_K779603 Version 1 (Component)
Hef1a-eYFP-4xFF1 MammoBlock Device

BBa_J92001
BBa_J92001 Version 1 (Component)
Lead Remover and Reporter Device

pTetR-LacI
BBa_I763030 Version 1 (Component)
LacI coding device regulated by pTetR

BBa_J37021
BBa_J37021 Version 1 (Component)
pTetR Transfer Function with aTc

BBa_K299029
BBa_K299029 Version 1 (Component)
RBS measurement device pT7 J61127 GFP

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

BBa_I763003
BBa_I763003 Version 1 (Component)
GFP coding device switched on by IPTG

BBa_K1606010
BBa_K1606010 Version 1 (Component)
Kumamax enzyme that gluten updated m-cherry reporter device

BBa_K511905
BBa_K511905 Version 1 (Component)
Repressible rtTA3 Transactivator Generator (Hef1a-LacO-rtTA3) MammoBlock Device

BBa_K1341011
BBa_K1341011 Version 1 (Component)
OR LOGIC GATE IN Graph Theory (GFP OUTPUT DEVICE)

BBa_K137033
BBa_K137033 Version 1 (Component)
Device with GFP with (AC)21 repeat after start codon

pCMV-ECFP-
BBa_I763023 Version 1 (Component)
LacI coding device with ECFP as a reporter regulated by pCMV

BBa_J04431
BBa_J04431 Version 1 (Component)
GFP Coding Device with promoter, RBS, GFP with LVA tag, and Terminator

BBa_K2123117
BBa_K2123117 Version 1 (Component)
Novel RFP device regulated by mercury: MerR (regulatory protein) + Stationary phase with mer operato

Adapter Bi
BBa_K1807015 Version 1 (Component)
This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.