BBa_K112211BBa_K112211 Version 1 (Component){a~int!} The integrase gene with rbs ready to be attached with stop codon, BBb format
BBa_K1332003BBa_K1332003 Version 1 (Component)The 5?? side of the intron(+exon fragment) from td gene of T4 phage without stop codon
xisBBa_K112204 Version 1 (Component){a~xis!} The bacteriophage lambda xis gene ready to have rbs attached and stop codon; assembly stand
BBa_K1848001BBa_K1848001 Version 1 (Component)Human Gut Hormone Glucagon-like peptide 1 (7-37) (Sequence Lacks Stop Codon, but part possesses it)
BBa_K209000BBa_K209000 Version 1 (Component)cAR1 (Dictyostelium discoideum), no STOP
BBa_K1051356BBa_K1051356 Version 1 (Component)K1051301(clb2 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 term
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.