ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

GFP
BBa_E0040 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

Amp Cas
BBa_I13800 Version 1 (Component)
Ampicillin Resistance Cassette

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

Cas9
Cas9 Version 1 (Component)
Organism: Cryptococcus neoformans var. grubi H99

BBa_K1773003
BBa_K1773003 Version 1 (Component)
I-F type CRISPR-Cas Cas3 gene

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

dCas 9
BBa_K1051800 Version 1 (Component)
protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.

BBa_E0044
BBa_E0044 Version 1 (Component)
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)

BBa_K1773005
BBa_K1773005 Version 1 (Component)
I-F type CRISPR-Cas Cascade complex

Cat
BBa_I14033 Version 1 (Component)
P(Cat)

phlF
BBa_K1725040 Version 1 (Component)
<i>phlF</i> encoding PhlF repressor from Pseudomonas protegens Pf-5

BBa_K1445001
BBa_K1445001 Version 1 (Component)
Endogenous Type II CRISPR-Cas9 phagemid

BBa_K1559002
BBa_K1559002 Version 1 (Component)
rearranged CRISPR/Cas9 system without promoter

GFP
GFP Version 1 (Component)
Organism: Cryptococcus neoformans serotype A H99S

BBa_K1218011
BBa_K1218011 Version 1 (Component)
Cas9

BBa_K1773002
BBa_K1773002 Version 1 (Component)
I-F type CRISPR-Cas Cas3 protein

Cse1
BBa_K1218003 Version 1 (Component)
CRISPR CasA E. coli (Modern)

BBa_K1014001
BBa_K1014001 Version 1 (Component)
HrpR Gene

BBa_K786033
BBa_K786033 Version 1 (Component)
cascade proteins, which consists of casA, casB, casC, casD, casE

Las CIO
BBa_I14016 Version 1 (Component)
P(Las) CIO

BBa_K1051008
BBa_K1051008 Version 1 (Component)
S. cerevisiae CAA terminator with stopcodon

scar
BBa_G0000 Version 1 (Component)
SpeI/XbaI scar for RBS-CDS junctions

BBa_I750016
BBa_I750016 Version 1 (Component)
Gas Vesicle polycistonic gene

BBa_K1773000
BBa_K1773000 Version 1 (Component)
I-F type CRISPR-Cas Cascade complex

BBa_K1773004
BBa_K1773004 Version 1 (Component)
I-F type CRISPR-Cas homogenous CRISPR region

BBa_K1014000
BBa_K1014000 Version 1 (Component)
HrpS Gene

BBa_K1773001
BBa_K1773001 Version 1 (Component)
I-F type CRISPR-Cas Cascade complex

BBa_K1315003
BBa_K1315003 Version 1 (Component)
The <i>rpfG</i> gene from <i>Xanthomonas campestris</i>

BBa_K861042
BBa_K861042 Version 1 (Component)
FadA, gene for fatty acid degradation from <i>Salmonella enterica</i> LT2

BBa_K1315005
BBa_K1315005 Version 1 (Component)
The <i>clp</i> gene from <i>Xanthomonas campestris</i>

OriTF
BBa_J01002 Version 1 (Component)
OriT-F (Origin of Transfer for the F-plasmid nic region)

BBa_J33201
BBa_J33201 Version 1 (Component)
E. coli chromosomal ars promoter with arsR repressor gene

BBa_K1632011
BBa_K1632011 Version 1 (Component)
fimE (wild-type)

BBa_K1632010
BBa_K1632010 Version 1 (Component)
fimB (wild-type)

BBa_K861031
BBa_K861031 Version 1 (Component)
FadJ, gene for fatty acid degradation from <i>E.coli str. K12</i>

BBa_K117000
BBa_K117000 Version 1 (Component)
Lysis gene (promotes lysis in colicin-producing bacteria strain)

BBa_K094100
BBa_K094100 Version 1 (Component)
cheZ gene

BBa_K325210
BBa_K325210 Version 1 (Component)
Red Firefly Luciferase and LRE<BR><i>L. Cruciata<BR>(E. coli optimised)</i>

BBa_K091121
BBa_K091121 Version 1 (Component)
LacI wild-type gene

BBa_K1465301
BBa_K1465301 Version 1 (Component)
<i>adhA</i> gene from <i>Lactococcus&nbsp;lactis</i>

BBa_K602000
BBa_K602000 Version 1 (Component)
<i>Deinococcus radiodurans</i> PprI