BBa_K2122000
BBa_K2122000 Version 1 (Component)
Device expressing the Gb3 Synthase enzyme under control of an arabinose inducible promoter

BBa_I746008
BBa_I746008 Version 1 (Component)
I0500 (Inducible pBad/araC) + I746001 (AIP generator - agrB + agrD with RBSes + Terminator)

BBa_K2170141
BBa_K2170141 Version 1 (Component)
Tetracycline inducible Outer membrane protein A signal peptide (TetR_TetO_OmpA) in RFC[25] N-part

BBa_K575047
BBa_K575047 Version 1 (Component)
Genomic RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP, Cons. Promoter + RBS (B0034) + RhlR

BBa_K575046
BBa_K575046 Version 1 (Component)
Genomic RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0034) + RhlR

BBa_K1952007
BBa_K1952007 Version 1 (Component)
Hydrazine Synthase subunit beta (Kust-2860) with strong RBS under lactose-inducible promoter

BBa_K1985013
BBa_K1985013 Version 1 (Component)
Sequence coding for Sup35 with residues 1-61 with an arabinose inducible promoter

BBa_K511825
BBa_K511825 Version 1 (Component)
Inducible Notch-Gal4-ESN Receptor Generator (UAS-Gal4-Notch-Gal4-ESN) MammoBlock Device

BBa_K511818
BBa_K511818 Version 1 (Component)
Inducible Notch-Gal4-ESN Receptor Generator (TRE-Tight-Notch-Gal4-ESN) MammoBlock Device

BBa_K1351025
BBa_K1351025 Version 1 (Component)
P_<i>comAB</i>, a CSP inducible promoter derived from <i>Streptococcus pneumoniae</i>

BBa_K1351024
BBa_K1351024 Version 1 (Component)
P_<i>comC</i>, a CSP inducible promoter derived from <i>Streptococcus pneumoniae</i>

BBa_K1199050
BBa_K1199050 Version 1 (Component)
LinA gamma-HCH->1,3,4,6-tetrachloro-1,4-cyclohexadiene, lacking stop coden

BBa_J147001
BBa_J147001 Version 1 (Component)
RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter

BBa_J147000
BBa_J147000 Version 1 (Component)
RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter

BBa_J147002
BBa_J147002 Version 1 (Component)
RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter

BBa_K1189020
BBa_K1189020 Version 1 (Component)
Light chain human ferritin with his-tag and E-coil under the lacI inducible promoter

BBa_K1189019
BBa_K1189019 Version 1 (Component)
Heavy chain human ferritin with his-tag and E-coil under the lacI inducible promoter

BBa_K511826
BBa_K511826 Version 1 (Component)
Inducible N-Cadherin-2A-EYFP Generator (UAS-Gal4-N-Cadherin-2A-EYFP) MammoBlock Device

BBa_K1363201
BBa_K1363201 Version 1 (Component)
Anti-LPS factor(LALF) regulated by IPTG

BBa_J36849
BBa_J36849 Version 1 (Component)
Lac-inducible generator of Lpp-OmpA(46-66)-Streptavidin single-chain dimeric + His6 tag

BBa_J36851
BBa_J36851 Version 1 (Component)
Lac-inducible generator of Lpp-OmpA(46-159)-Streptavidin single-chain dimeric + His6 tag

BBa_K1985014
BBa_K1985014 Version 1 (Component)
Sequence coding for Sup35(residues 1-61) and Cytochrome b562 with an arabinose inducible promoter

BBa_K175050
BBa_K175050 Version 1 (Component)
aTc inducible GFP-LVA generator with integrated I-SceI RS + I-SceI RS + TetR generator

BBa_K831014
BBa_K831014 Version 1 (Component)
Inducible istR (inhibitor of SOS-induced toxicity by RNA) under the control of lac promoter

BBa_K1189030
BBa_K1189030 Version 1 (Component)
TALE-B with a his6 tag linked to a K coil under an inducible lacI promoter

BBa_K1199047
BBa_K1199047 Version 1 (Component)
LinA-RBS-LinB gamma-HCH->2,5-dichloro-2,5-cyclohexadiene-1,4-diol

BBa_I763003
BBa_I763003 Version 1 (Component)
GFP coding device switched on by IPTG

BBa_K175035
BBa_K175035 Version 1 (Component)
Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock

BBa_K175034
BBa_K175034 Version 1 (Component)
Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock

BBa_I13035
BBa_I13035 Version 1 (Component)
3OC₆HSL Receiver Device with Inducible Control of LuxR and a YFP Output device

BBa_K1955010
BBa_K1955010 Version 1 (Component)
pSB1A2-LacI inducible GFP

BBa_K944001
BBa_K944001 Version 1 (Component)
Transcriptional Regulator for Cyanide Inducible Promoter

BBa_K1954007
BBa_K1954007 Version 1 (Component)
Green Light Inducible bacteriocin Device (GLID)

BBa_K1323019
BBa_K1323019 Version 1 (Component)
Hfq expression cassette under a xylose inducible promoter

BBa_K2092002
BBa_K2092002 Version 1 (Component)
PalcA, improved alcR inducible promoter from A. nidulans

BBa_K590046
BBa_K590046 Version 1 (Component)
AAR-PSB3K3-Lac Inducible w/o LacI

BBa_K638201
BBa_K638201 Version 1 (Component)
Arabinose inducible Poly-His Reflectin A1 generator

AraC RBS
BBa_K1392935 Version 1 (Component)
AraC-Pbad - Arabinose inducible regulatory promoter/repressor unit+ RBS

BBa_K1968009
BBa_K1968009 Version 1 (Component)
PglaA inducible promoter Phytobrick: glucoamylase gene promoter (PglaA) from Aspergillus niger

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

BBa_K563053
BBa_K563053 Version 1 (Component)
vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.