BBa_K1405007
BBa_K1405007 Version 1 (Component)
A Kill Switch with "memory" time repressed by IPTG

BBa_I13306
BBa_I13306 Version 1 (Component)
R0040.I13279 Tet-Regulated Las Amplifier

BBa_K1363200
BBa_K1363200 Version 1 (Component)
Anti-LPS factor(LALF) regulated by lacI

BBa_K594014
BBa_K594014 Version 1 (Component)
A device that can accepts the 3--OH-C14:1-HSL and then produces 3-O-C6-HSL and GFP reporter.

BBa_K1695049
BBa_K1695049 Version 1 (Component)
pL8-UV5 + Riboswitch Bacteriophage 21 Codon Optimized Lysis Cassette S R Rz

BBa_F2710
BBa_F2710 Version 1 (Component)
N-butyryl-HSL Receiver Device

Plac-LacY-
BBa_I763014 Version 1 (Component)
LacY and cI regulated by PLac Promoter

BBa_K1088057
BBa_K1088057 Version 1 (Component)
T25 domain of bacterial two-hybrid system (IPTG inducible)

BBa_K1954007
BBa_K1954007 Version 1 (Component)
Green Light Inducible bacteriocin Device (GLID)

BBa_K779603
BBa_K779603 Version 1 (Component)
Hef1a-eYFP-4xFF1 MammoBlock Device

BBa_J92001
BBa_J92001 Version 1 (Component)
Lead Remover and Reporter Device

pSBBs0K
BBa_K823026 Version 1 (Component)
pSB_Bs0K-P_spac (replicative Bacillus subtilis expression vector; IPTG inducible

BBa_K299029
BBa_K299029 Version 1 (Component)
RBS measurement device pT7 J61127 GFP

P5+E0240
BBa_K100007 Version 1 (Component)
Edited Xylose Regulated Bi-Directional Operator 3 + GFP

BBa_K196006
BBa_K196006 Version 1 (Component)
Promoter (lacI regulated, lambda pL hybrid) + RBS (Elowitz 1999)

BBa_K944001
BBa_K944001 Version 1 (Component)
Transcriptional Regulator for Cyanide Inducible Promoter

CsgD
BBa_K1019001 Version 1 (Component)
CsgD: positive regulator of curlin genes

BBa_K1606010
BBa_K1606010 Version 1 (Component)
Kumamax enzyme that gluten updated m-cherry reporter device

BBa_K511905
BBa_K511905 Version 1 (Component)
Repressible rtTA3 Transactivator Generator (Hef1a-LacO-rtTA3) MammoBlock Device

BBa_K1341011
BBa_K1341011 Version 1 (Component)
OR LOGIC GATE IN Graph Theory (GFP OUTPUT DEVICE)

BBa_K584008
BBa_K584008 Version 1 (Component)
Lambda cI and LuxR regulated hybrid promotor + RBS + MelA + RBS + AFP + term

Adapter Bi
BBa_K1807000 Version 1 (Component)
Protein generator device suitable for blue-white screening and Gibson Assembly.

BBa_K2144011
BBa_K2144011 Version 1 (Component)
Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter

BBa_K137033
BBa_K137033 Version 1 (Component)
Device with GFP with (AC)21 repeat after start codon

BBa_K581003
BBa_K581003 Version 1 (Component)
SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)

BBa_K549004
BBa_K549004 Version 1 (Component)
LacI promotor fused with the iron dependent regulator fur

BBa_J04431
BBa_J04431 Version 1 (Component)
GFP Coding Device with promoter, RBS, GFP with LVA tag, and Terminator

BBa_I741016
BBa_I741016 Version 1 (Component)
Reverse Total XylR Transcriptional Regulator Left Facing (I741011 reverse complement

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.