BBa_J04430BBa_J04430 Version 1 (Component)GFP coding device switched on by IPTG
BBa_I746008BBa_I746008 Version 1 (Component)I0500 (Inducible pBad/araC) + I746001 (AIP generator - agrB + agrD with RBSes + Terminator)
BBa_K133005BBa_K133005 Version 1 (Component)CF-UreB-RGD-Histop
BBa_J29015BBa_J29015 Version 1 (Component)arabinose induable CrtE CrtB CrtI generator
BBa_K2170141BBa_K2170141 Version 1 (Component)Tetracycline inducible Outer membrane protein A signal peptide (TetR_TetO_OmpA) in RFC[25] N-part
BBa_K575047BBa_K575047 Version 1 (Component)Genomic RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP, Cons. Promoter + RBS (B0034) + RhlR
BBa_K575046BBa_K575046 Version 1 (Component)Genomic RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0034) + RhlR
BBa_K1952007BBa_K1952007 Version 1 (Component)Hydrazine Synthase subunit beta (Kust-2860) with strong RBS under lactose-inducible promoter
BBa_K1985013BBa_K1985013 Version 1 (Component)Sequence coding for Sup35 with residues 1-61 with an arabinose inducible promoter
BBa_K511825BBa_K511825 Version 1 (Component)Inducible Notch-Gal4-ESN Receptor Generator (UAS-Gal4-Notch-Gal4-ESN) MammoBlock Device
BBa_K511818BBa_K511818 Version 1 (Component)Inducible Notch-Gal4-ESN Receptor Generator (TRE-Tight-Notch-Gal4-ESN) MammoBlock Device
BBa_J29010BBa_J29010 Version 1 (Component)lac I and arabinose induable CtrI generator
BBa_K1075027BBa_K1075027 Version 1 (Component)SplitSspB(Rapamycin inducable)-pLac-RBS34-mCherry-(Ec)ssrA(DAS+4)-TT
BBa_K1351025BBa_K1351025 Version 1 (Component)P<sub><i>comAB</i></sub>, a CSP inducible promoter derived from <i>Streptococcus pneumoniae</i>
BBa_K1351024BBa_K1351024 Version 1 (Component)P<sub><i>comC</i></sub>, a CSP inducible promoter derived from <i>Streptococcus pneumoniae</i>
BBa_J147001BBa_J147001 Version 1 (Component)RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter
BBa_K133039BBa_K133039 Version 1 (Component)T7-CF-UREB-RGD-Histop
BBa_J147000BBa_J147000 Version 1 (Component)RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter
BBa_J147002BBa_J147002 Version 1 (Component)RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter
BBa_K1351039BBa_K1351039 Version 1 (Component)P<sub><i>xylA</i></sub>, a xylose inducible promoter for <i>Bacillus subtilis</i>
BBa_K1189020BBa_K1189020 Version 1 (Component)Light chain human ferritin with his-tag and E-coil under the lacI inducible promoter
BBa_K1189019BBa_K1189019 Version 1 (Component)Heavy chain human ferritin with his-tag and E-coil under the lacI inducible promoter
BBa_K511826BBa_K511826 Version 1 (Component)Inducible N-Cadherin-2A-EYFP Generator (UAS-Gal4-N-Cadherin-2A-EYFP) MammoBlock Device
BBa_J36849BBa_J36849 Version 1 (Component)Lac-inducible generator of Lpp-OmpA(46-66)-Streptavidin single-chain dimeric + His6 tag
BBa_J36851BBa_J36851 Version 1 (Component)Lac-inducible generator of Lpp-OmpA(46-159)-Streptavidin single-chain dimeric + His6 tag
BBa_K1985014BBa_K1985014 Version 1 (Component)Sequence coding for Sup35(residues 1-61) and Cytochrome b562 with an arabinose inducible promoter
BBa_K175050BBa_K175050 Version 1 (Component)aTc inducible GFP-LVA generator with integrated I-SceI RS + I-SceI RS + TetR generator
BBa_K831014BBa_K831014 Version 1 (Component)Inducible istR (inhibitor of SOS-induced toxicity by RNA) under the control of lac promoter
BBa_K1189030BBa_K1189030 Version 1 (Component)TALE-B with a his6 tag linked to a K coil under an inducible lacI promoter
BBa_K175035BBa_K175035 Version 1 (Component)Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock
BBa_K175034BBa_K175034 Version 1 (Component)Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock
BBa_K242999BBa_K242999 Version 1 (Component)Colicin E3 rRNAse domain
BBa_K1363201BBa_K1363201 Version 1 (Component)Anti-LPS factor(LALF) regulated by IPTG
BBa_I13035BBa_I13035 Version 1 (Component)3OC<sub>6</sub>HSL Receiver Device with Inducible Control of LuxR and a YFP Output device
BBa_M45102BBa_M45102 Version 1 (Component)Cobalt detection Biobrick. RFP made in presence of Cobalt. Note the receptor also works in the prese
BBa_I763003BBa_I763003 Version 1 (Component)GFP coding device switched on by IPTG
BBa_K1955010BBa_K1955010 Version 1 (Component)pSB1A2-LacI inducible GFP
BBa_K944001BBa_K944001 Version 1 (Component)Transcriptional Regulator for Cyanide Inducible Promoter
BBa_K1954007BBa_K1954007 Version 1 (Component)Green Light Inducible bacteriocin Device (GLID)
BBa_K1323019BBa_K1323019 Version 1 (Component)Hfq expression cassette under a xylose inducible promoter
BBa_K2092002BBa_K2092002 Version 1 (Component)PalcA, improved alcR inducible promoter from A. nidulans
BBa_K590046BBa_K590046 Version 1 (Component)AAR-PSB3K3-Lac Inducible w/o LacI
BBa_K638201BBa_K638201 Version 1 (Component)Arabinose inducible Poly-His Reflectin A1 generator
BBa_K1968009BBa_K1968009 Version 1 (Component)PglaA inducible promoter Phytobrick: glucoamylase gene promoter (PglaA) from Aspergillus niger
AraC_TEV-FBBa_K627008 Version 1 (Component)Fusion part of arabinose-inducible induction system and the TEV protease
BBa_K563053BBa_K563053 Version 1 (Component)vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.
BBa_K1075003BBa_K1075003 Version 1 (Component)Promoter(const.)-AraC-Term-pBAD-RBS34 (Arabinose inducable promoter system)
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.