BBa_K1039024BBa_K1039024 Version 1 (Component)PhiC31 integrase under lock and key control with lacI expression cassette
BBa_K112212BBa_K112212 Version 1 (Component){rbs.int!} The integrase gene with native rbs site and stop codon, BBb format
BBa_K2116028BBa_K2116028 Version 1 (Component)bxb1 integrase under the control of nitric oxide and AHL responsive AND gate
BBa_K1510931BBa_K1510931 Version 1 (Component)Sequence integrated the luxR gene and pluxR promoter together.
BBa_K112211BBa_K112211 Version 1 (Component){a~int!} The integrase gene with rbs ready to be attached with stop codon, BBb format
BBa_K1039022BBa_K1039022 Version 1 (Component)PhiC31 integrase and PhiC31 RDF under lock and key control with lacI Expression Cassette
BBa_K1039021BBa_K1039021 Version 1 (Component)Bxb1 integrase and Recombination Directionality Factor (RDF) Under ???Lock and Key??? Control with L
BBa_K863205BBa_K863205 Version 1 (Component)GFP integrated in shuttle vector pECPP11JS (BBa_K863204) for integration in yeast
BBa_K863209BBa_K863209 Version 1 (Component)BBa_500002 integrated in shuttle vector pECPP11JS (BBa_K863204) for integration in yeast
BBa_K863208BBa_K863208 Version 1 (Component)tvel35 integrated in shuttle vector pECPP11JS (BBa_K863204) for integration in yeast
BBa_K863207BBa_K863207 Version 1 (Component)tvel5 integrated in shuttle vector pECPP11JS (BBa_K863204) for integration in yeast
MtrBBBa_K1748001 Version 1 (Component)Extracelllular metal reducing respiratory system integral outer-membrane β-barrel protein MtrB
BBa_K175050BBa_K175050 Version 1 (Component)aTc inducible GFP-LVA generator with integrated I-SceI RS + I-SceI RS + TetR generator
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.