BBa_K1916008
BBa_K1916008 Version 1 (Component)
Osc7112_5903g3 + intein-CBD tag

BBa_K1916010
BBa_K1916010 Version 1 (Component)
CBCR2 + intein-CBD tag

BBa_K1916023
BBa_K1916023 Version 1 (Component)
CBCR11 + intein-CBD tag

BBa_K1916017
BBa_K1916017 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916015
BBa_K1916015 Version 1 (Component)
CBCR4 + intein-CBD tag

BBa_K1916020
BBa_K1916020 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916019
BBa_K1916019 Version 1 (Component)
CBCR8 + intein-CBD tag

BBa_K1916009
BBa_K1916009 Version 1 (Component)
Osc7112_5903g3 + intein-CBD tag

BBa_K1916001
BBa_K1916001 Version 1 (Component)
NpF2164g5 + intein-CBD tag

BBa_K1916018
BBa_K1916018 Version 1 (Component)
CBCR8 + intein-CBD tag

BBa_K1916013
BBa_K1916013 Version 1 (Component)
CBCR3 + intein-CBD tag

BBa_K1916007
BBa_K1916007 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1916014
BBa_K1916014 Version 1 (Component)
CBCR4 + intein-CBD tag

BBa_K1916000
BBa_K1916000 Version 1 (Component)
NpF2164g5 + intein-CBD tag

BBa_K1916003
BBa_K1916003 Version 1 (Component)
Cha6605_4344g2 + intein-CBD tag

BBa_K1916006
BBa_K1916006 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1435000
BBa_K1435000 Version 1 (Component)
4-HT inducible intein

BBa_K1916002
BBa_K1916002 Version 1 (Component)
Cyan7427_1390g3 + intein-CBD tag

BBa_K1916021
BBa_K1916021 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916004
BBa_K1916004 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916016
BBa_K1916016 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916012
BBa_K1916012 Version 1 (Component)
CBCR3 + intein-CBD tag

BBa_K1319016
BBa_K1319016 Version 1 (Component)
TEV protease cleavage site

BBa_J70628
BBa_J70628 Version 1 (Component)
RFC12 Sce VMA Intein Internal Domain

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

CFY
BBa_K1381017 Version 1 (Component)
CFY-X6 his

BBa_K1896005
BBa_K1896005 Version 1 (Component)
mSA2-His

7x His
BBa_T2001 Version 1 (Component)
7x His tag head domain

BBa_K362001
BBa_K362001 Version 1 (Component)
ahpC, this promoter is controlled by the OxyR protein.

BBa_K1189009
BBa_K1189009 Version 1 (Component)
Beta-lactamase with His Tag

BBa_I712043
BBa_I712043 Version 1 (Component)
CMVp - myristoilation site - HIV protease cleavage site - T7RNAP - NLS

BBa_I712044
BBa_I712044 Version 1 (Component)
CMVp - myristoilation site - HIV protease cleavage site - mCherry - NLS

BBa_K748002
BBa_K748002 Version 1 (Component)
Truncated lysostaphin coding sequence. Lysostaphin has has a specific lytic action against S.aureus.

RGK
BBa_K1362434 Version 1 (Component)
RGK (extein)

7x His
BBa_T2000 Version 1 (Component)
7x His tag tail domain

BBa_K1679022
BBa_K1679022 Version 1 (Component)
KIG, kcoil+intein+gfp

BBa_K1679027
BBa_K1679027 Version 1 (Component)
VMA, a thermosensitive intein

tphC_322aa
BBa_K808001 Version 1 (Component)
tphC: terephtalate periplasmatic binding proteine of the tripartite transporter family

3xSWAG(N)
BBa_K1362900 Version 1 (Component)
N-terminal tripleSWAGtag (N-intein)

BBa_K1679037
BBa_K1679037 Version 1 (Component)
VMA Dead, an inactivated intein

BBa_K1362901
BBa_K1362901 Version 1 (Component)
C-terminal tripleSWAGtag (C-intein)

BBa_I712047
BBa_I712047 Version 1 (Component)
CMVp - HA - CD4 - HIV protease cleavage site - T7RNAP - NLS

AraC_14_3C
BBa_K627011 Version 1 (Component)
Fusion part of pBAD arabinose-inducible induction system and the HRV 14_3C protease

AraC_TEV-F
BBa_K627009 Version 1 (Component)
Arabinose inducible TEV protease

BBa_K1256006
BBa_K1256006 Version 1 (Component)
pSB1C3-Plac-SS-Protease

BBa_K1680011
BBa_K1680011 Version 1 (Component)
HCV protease with linkers

INP-His
BBa_K1933001 Version 1 (Component)
INPNC fused to 6xHis tag

BBa_K1319008
BBa_K1319008 Version 1 (Component)
IPTG-induced and T7-driven expression of TEV protease

BBa_K1491027
BBa_K1491027 Version 1 (Component)
T7 RNA Polymerase with Dead ER Intein